Investigating the distribution of HIV-1 Tat lengths present in the Drexel Medicine CARES cohort
| Autor: | Robert W. Link, Jeffrey M. Jacobson, Kim Malone, Gregory C. Antell, Will Dampier, Shendra Passic, Katherine Kercher, Anthony R. Mele, Brian Wigdahl, Wen Zhong, Zsofia Szep, Vanessa Pirrone, Michael R. Nonnemacher |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Gene Expression Regulation
Viral Transcriptional Activation Cancer Research Human immunodeficiency virus (HIV) HIV Infections Biology medicine.disease_cause Hiv 1 tat Article 03 medical and health sciences symbols.namesake Virology medicine Humans In patient Cells Cultured HIV Long Terminal Repeat 030304 developmental biology Sanger sequencing 0303 health sciences 030306 microbiology Stop codon CD4 Lymphocyte Count Infectious Diseases Viral replication Cohort Codon Terminator HIV-1 symbols tat Gene Products Human Immunodeficiency Virus National laboratory |
| Zdroj: | Virus Res |
| ISSN: | 0168-1702 |
| Popis: | Human immunodeficiency virus type 1 (HIV-1) encodes for Tat, a multi-functional regulatory protein involved in transcriptional enhancement and in causing neurotoxicity/central nervous system (CNS) dysfunction. This study examines Sanger sequencing of HIV-1 subtype B Tat from 2006 to 2014 within the Drexel University College of Medicine CNS AIDS Research and Eradication Study (CARES) Cohort to investigate Tat length in patients. The Los Alamos National Laboratory (LANL) database was used as a comparator. Miscoded stop codons were present in the CARES Cohort and LANL and protein variability was highly similar. Tat proteins in CARES and LANL were predominantly 101 residues. There was no observed correlation between Tat length and clinical parameters within the CARES Cohort. Unique Tat lengths found in the CARES Cohort and not in LANL were 31, 36, and 39 residues. When CARES patients were longitudinally examined, sequence lengths of 101 had a low probability of reducing to below 48, and sequences had a high probability of increasing to above 86 residues during their next visit, when below 48 residues in length. This suggests that Tat length is conserved to retain the majority of the proteins function highlighting its importance in viral replication. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect