Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult Rats
| Autor: | Rodrigo Yokota, Manassés C. Fonteles, Renata Oliveira Pereira, Fabiana S. Evangelista, Patricia Fiorino, Cynthia Rodrigues Muller, Nilberto R.F. Nascimento, Anna Laura Viacava Américo, Ana Paula Oliveira Leite, Vera Farah |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
kidney lipid deposition
0301 basic medicine medicine.medical_specialty Normal diet Endocrinology Diabetes and Metabolism medicine.medical_treatment Renal function lcsh:TX341-641 INFLAMAÇÃO 030209 endocrinology & metabolism Diet High-Fat Proinflammatory cytokine post-weaning 03 medical and health sciences Dieta Hiperlipídica 0302 clinical medicine Insulin resistance Internal medicine medicine Ingestion Lipídeos Nutrition Original Research Kidney 030109 nutrition & dietetics Nutrition and Dietetics business.industry lipotoxicity medicine.disease Lipids high-fat diet medicine.anatomical_structure Cytokine Endocrinology Lipotoxicity inflammation business lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Repositório Institucional da Universidade Federal do Ceará (UFC) Universidade Federal do Ceará (UFC) instacron:UFC Frontiers in Nutrition Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Frontiers in Nutrition, Vol 6 (2019) |
| ISSN: | 2296-861X |
| Popis: | Aim: We investigated the kidney morphofunctional consequences of high-fat diet intake since post-weaning in adult rats. Main Methods: Male Wistar rats were divided into two groups: ND (normal diet; n = 10) and HD (high-fat diet; n = 10). The high-fat diet was introduced post-weaned and animals were followed for 8 weeks. Key Findings: HD group did not change body weight gain even though food consumption has decreased with no changes in caloric consumption. The HD group showed glucose intolerance and insulin resistance. The glomerular filtration rate (GFR) was decreased in vivo (ND: 2.8 ± 1.01; HD: 1.1 ± 0.14 ml/min) and in the isolated perfusion method (34% of decrease). Renal histological analysis showed a retraction in glomeruli and an increase in kidney lipid deposition (ND: 1.5 ± 0.17 HD: 5.9 ± 0.06%). Furthermore, the high-fat diet consumption increased the pro-inflammatory cytokines IL-6 (ND: 1,276 ± 203; HD: 1,982 ± 47 pg/mL/mg) and IL-1b (ND: 97 ± 12 HD: 133 ± 5 pg/mL/mg) without changing anti-inflammatory cytokine IL-10. Significance: Our study provides evidence that high-fat diet consumption leads to renal lipid accumulation, increases inflammatory cytokines, induces glomeruli retraction, and renal dysfunction. These damages observed in the kidney could be associated with an increased risk to advanced CKD in adulthood suggesting that reduction of high-fat ingestion during an early period of life can prevent metabolic disturbances and renal lipotoxicity. |
| Databáze: | OpenAIRE |
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