Intravenously Injected Pluripotent Stem Cell–derived Cells Form Fetomaternal Vasculature and Prevent Miscarriage in Mouse

Autor: Kiichiro Tomoda, Masahide Ohmichi, Natsuko Morita, Atsushi Daimon, Yoshinori Koishi, Kazuyoshi Kanki, Michio Asahi, Hirofumi Morihara
Rok vydání: 2020
Předmět:
Male
Pluripotent Stem Cells
0301 basic medicine
Abortion
Habitual
induced pluripotent stem cells
medicine.medical_treatment
Biomedical Engineering
lcsh:Medicine
Neovascularization
Physiologic
Enzyme-Linked Immunosorbent Assay
stem cell therapy
Miscarriage
Cell therapy
Andrology
Mice
03 medical and health sciences
0302 clinical medicine
Recurrent miscarriage
medicine
Animals
Humans
Progenitor cell
Induced pluripotent stem cell
endothelial progenitor cells
Mice
Inbred BALB C
Transplantation
Microscopy
Confocal
business.industry
lcsh:R
digestive
oral
and skin physiology
cellular therapy
Cell Biology
Stem-cell therapy
embryonic stem cells
medicine.disease
Embryonic stem cell
digestive system diseases
030104 developmental biology
medicine.anatomical_structure
Microscopy
Fluorescence
Female
Original Article
Bone marrow
business
030217 neurology & neurosurgery
Zdroj: Cell Transplantation
Cell Transplantation, Vol 29 (2020)
ISSN: 1555-3892
0963-6897
DOI: 10.1177/0963689720970456
Popis: Miscarriage is the most common complication of pregnancy, and about 1% of pregnant women suffer a recurrence. Using a widely used mouse miscarriage model, we previously showed that intravenous injection of bone marrow (BM)-derived endothelial progenitor cells (EPCs) may prevent miscarriage. However, preparing enough BM-derived EPCs to treat a patient might be problematic. Here, we demonstrated the generation of mouse pluripotent stem cells (PSCs), propagation of sufficient PSC-derived cells with endothelial potential (PSC-EPs), and intravenous injection of the PSC-EPs into the mouse miscarriage model. We found that the injection prevented miscarriage. Three-dimensional reconstruction images of the decidua after tissue cleaning revealed robust fetomaternal neovascularization induced by the PSC-EP injection. Additionally, the injected PSC-EPs directly formed spiral arteries. These findings suggest that intravenous injection of PSC-EPs could become a promising remedy for recurrent miscarriage.
Databáze: OpenAIRE
Externí odkaz: