A novel HPLC-DAD method for simultaneous determination of febuxostat and diclofenac in biological samples: pharmacokinetic outcomes
| Autor: | Essam F. Khamis, Omayma A. Amin, Eman I. El-Kimary, Sameh E. Younis, Fawzy A. El-Yazbi, Mohammed A.W. Elkhatib, Ahmed F. El-Yazbi |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Diclofenac Liquid-Liquid Extraction Clinical Biochemistry Sensitivity and Specificity 030226 pharmacology & pharmacy 01 natural sciences Analytical Chemistry Rats Sprague-Dawley 03 medical and health sciences Febuxostat 0302 clinical medicine Pharmacokinetics medicine Animals Humans Tissue Distribution General Pharmacology Toxicology and Pharmaceutics Chromatography High Pressure Liquid Cross-Over Studies Chromatography Chemistry 010401 analytical chemistry Reproducibility of Results General Medicine Healthy Volunteers Rats 0104 chemical sciences Medical Laboratory Technology Liver Human plasma Area Under Curve Calibration Hplc dad medicine.drug |
| Zdroj: | Bioanalysis. 11:41-54 |
| ISSN: | 1757-6199 1757-6180 |
| DOI: | 10.4155/bio-2018-0219 |
| Popis: | Aim: To develop a simple HPLC-DAD method for simultaneous determination of febuxostat (FEB) and diclofenac (DIC) in biological samples to assess pharmacokinetic outcomes of their coadministration. Methodology & results: Sample preparation was performed by liquid–liquid extraction. Drugs analysis was done on C18 column using methanol-formic acid pH 2.1 (76:24, v/v) as mobile phase and time-programmed UV detection. Lower limits of quantitation for FEB and DIC were 10 and 20 ng/ml, respectively. Baseline pharmacokinetics were similar to published data on either drug alone. Coadministration led to more than twofold increase in FEB Cmax and AUC together with a reduced hepatic uptake in rats. Conclusion: DIC interfered with initial distribution and terminal clearance of FEB potentially due to reduced FEB hepatic uptake. |
| Databáze: | OpenAIRE |
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