A SURVEY OF TRANSCRIPTS EXPRESSED SPECIFICALLY IN ROOT-NODULES OF BROADBEAN (VICIA-FABA L)
| Autor: | Andreas M. Perlick, Alfred Pühler |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
DNA
Complementary Transcription Genetic Lipoxygenase Molecular Sequence Data Asparagine synthetase Plant Science Biology Homology (biology) Gene product Peptide Elongation Factor 1 Nitrogen Fixation Complementary DNA Gene expression Genetics Amino Acid Sequence RNA Messenger Cloning Molecular Gene NODULE-SPECIFIC GENE EXPRESSION Gene Library Plant Proteins DIFFERENTIAL CDNA LIBRARY Plants Medicinal cDNA library RNA Fabaceae NODULINS General Medicine VICIA-FABA L Blotting Northern Peptide Elongation Factors Molecular biology Proline-Rich Protein Domains Peptides Agronomy and Crop Science |
| DOI: | 10.1007/bf00028969 |
| Popis: | More than 600 potentially nodule-specific clones have been detected by differential hybridization of a broadbean cDNA library constructed from root nodule poly(A)+ RNA. These isolated cDNAs belong to at least 28 different clone groups containing cross-hybridizing sequences. The number of clones within a clone group varies from about 200 to only one single clone. Northern hybridization experiments revealed nodule-specific transcripts for 14 clone groups and markedly nodule-enhanced transcripts for another 7 clone groups. Sequence homologies indicate that three transcript sequences code for different leghemoglobins. Two other transcripts encode a nodule-specific sucrose synthase and a nodule-enhanced asparagine synthetase, respectively. Four deduced gene products are proline-rich, two of them being the homologues of PsENOD2 and PsENOD12. The third proline-rich protein (PRP) is composed of similar amino acid repeats as the nodule-specific PsENOD12 but is expressed in nodules and roots in comparable amounts. The fourth PRP is a nodule-enhanced extensin-type protein built up by Ser-Pro4 repeats. Two further nodule-specific transcripts encode gene products showing some similarity to structural glycine-rich proteins. Additionally, transcripts could be identified for broadbean homologues of the nodulins MsNOD25, PsENOD3 and PsENOD5 and transcripts specifying a nodule-enhanced lipoxygenase and a translation elongation factor EF-1 alpha, which is expressed in all broadbean tissues tested. |
| Databáze: | OpenAIRE |
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