Calpain-calpastatin system of canine basilar artery in vasospasm
| Autor: | Takaomi C. Saido, Yukio Maeda, Eiichi Tani, Koichi Suzuki, Nobutaka Minami, Ikuya Yamaura |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Serotonin
medicine.medical_specialty Contraction (grammar) Immunoblotting In Vitro Techniques Potassium Chloride Tonic (physiology) Dogs medicine.artery Internal medicine medicine Basilar artery Carnivora Animals Calpastatin biology Calpain business.industry Calcium-Binding Proteins Fissipedia Vasospasm Anatomy medicine.disease biology.organism_classification Endocrinology Ischemic Attack Transient Basilar Artery biology.protein business |
| Zdroj: | Journal of Neurosurgery. 79:537-543 |
| ISSN: | 0022-3085 |
| DOI: | 10.3171/jns.1993.79.4.0537 |
| Popis: | ✓ Vasospasm was produced in the canine basilar artery by a two-hemorrhage method, while contraction was induced in the normal canine basilar artery by a local application of KCl or serotonin after transclival exposure. The control animals were injected with saline instead of fresh blood. The activation of μ-calpain, a Ca++-dependent neutral protease, in the basilar artery was studied by evaluating the conversion from its inactivated into its activated form on immunoblots. In addition, the activity of calpastatin, an intrinsic inhibitor of calpain, in the basilar artery was determined by assay. The majority of the μ-calpain was inactivated in the control group. In the spastic group, μ-calpain was generally activated markedly in the early stage of vasospasm and moderately thereafter. The contraction induced by KCl or serotonin application was classified into the early phasic and the later tonic stages; μ-calpain was usually activated in the phasic stage and inactivated in the tonic stage. Calpastatin activity was significantly decreased during vasospasm, whereas it was not significantly changed in KCl- or serotonin-induced contraction. The final activity of μ-calpain results from the balance of μ-calpain and calpastatin. This suggests that μ-calpain activity was enhanced continuously in the spastic group and transiently in the KCl or serotonin group, and that the continuous activation of μ-calpain during vasospasm probably induced more proteolytic changes compared to those in the KCl or serotonin group. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|