Storage of washed or irradiated red cells in AS ‐7 improves their in vitro characteristics
| Autor: | S. Thomas, David J. Smith, M A Popovsky, Rebecca Cardigan, David J. Roberts, I Swann, S. Proffitt |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Erythrocytes Time Factors Sodium Chloride urologic and male genital diseases Shelf life Hemolysis Andrology Adenosine Triphosphate medicine Humans Mannitol Irradiation neoplasms Red Cell Chemistry Adenine Hematology General Medicine Hydrogen-Ion Concentration Haemolysis female genital diseases and pregnancy complications In vitro Surgery Glucose Blood Preservation Gamma Rays Blood processing |
| Zdroj: | Vox Sanguinis. 109:203-213 |
| ISSN: | 1423-0410 0042-9007 |
| Popis: | Background AS-7 is a new alkaline hypotonic red cell additive solution (AS) shown to improve red cell quality during storage compared with AS-1. We sought to compare red cells stored in AS-7 with those stored in SAGM using RCC that were either untreated, or washed or irradiated on day 14 of storage. Study Design and Methods A pooled and split study design was used to produce seven identical RCC (four in SAGM and three in AS-7). At day 14 following donation, two RCC (one in SAGM and one in AS-7) were gamma irradiated and three RCC (two in SAGM and one in AS-7) were washed and resuspended in either SAGM or AS-7. RCC were sampled for analysis throughout storage and at end of shelf life: day 28 for washed or irradiated and day 35 for untreated RCC. Results For untreated, washed or irradiated RCC, those stored in AS-7 had lower haemolysis, red cell microvesicles and supernatant potassium content than RCC in SAGM. In addition, ATP levels and pH were better maintained in AS-7 RCC than in SAGM RCC. Conclusion These data suggest that the quality of these components may be improved by storage in AS-7 compared with SAGM. |
| Databáze: | OpenAIRE |
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