Antifungal activity of Saccharomyces cerevisiae and assessment of ochratoxigenic load on currants by means of Real Time PCR
| Autor: | Paraskevi Kaplani, Efstathios Z. Panagou, Leonidas Skarlatos, Paschalitsa Tryfinopoulou |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Ochratoxin A
Fusarium Antifungal Agents Saccharomyces cerevisiae Real-Time Polymerase Chain Reaction Microbiology 03 medical and health sciences chemistry.chemical_compound Ribes Yeast Dried Antibiosis Food science Ochratoxin 030304 developmental biology 0303 health sciences biology 030306 microbiology Penicillium Alternaria General Medicine biology.organism_classification Ochratoxins Yeast Aspergillus chemistry Fruit Cladosporium Food Science |
| Zdroj: | International Journal of Food Microbiology. 344:109111 |
| ISSN: | 0168-1605 |
| Popis: | Currants are prone to contamination by ochratoxin during cultivation, processing and storage conditions. Saccharomyces cerevisiae is considered to be among the main species of grape yeast flora able to control antagonistic fungi. In this study, the potential of S. cerevisiae Y33 was investigated to inhibit the growth of several fungal species indigenous to the microbiota of grapes. Moreover, the efficacy of this yeast species was investigated to inhibit OTA by toxin producing fungi both in vitro and in situ. For this purpose thirty-five different fungal species, belonging to the genera Aspergillus, Penicillium, Cladosporium, Fusarium and Alternaria interacted in vitro with S. cerevisiae on Malt Extract agar plates, stored at 25 °C for 14 days. Results showed that the highest OTA producer A. carbonarius F71 was inhibited more than 99% from day 7, in contrast to A. niger strains that presented enhanced OTA production at day 14 due to interaction with S. cerevisiae Y33. Additionally, the antifungal potential of the selected yeast was also studied in situ on currants subjected to different treatments and stored at 25 °C for 28 days. Microbiological analysis was undertaken for the enumeration of the bacterial and fungal flora, together with OTA determination at 7 and 21 days. To quantify A. carbonarius on all treated currant samples, molecular analysis with Real Time PCR was employed. A standard curve was prepared with A. carbonarius DNA. The efficiency of the curve was estimated to 10.416, the slope to −3.312 and the range of haploid genome that could be estimated was from 1.05 to 105∙105. The amount of A. carbonarius DNA in all treated currants samples, where the fungus was positively detected, ranged from as low as 0.08 to 562 ng DNA/g currants. The antifungal activity of S. cerevisiae Y33 was observed in all studied cases, causing inhibition of fungal growth and OTA production. |
| Databáze: | OpenAIRE |
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