Reabsorption of lung liquid produced by 2,4-dinitrophenol in in vitro lungs from fetal guinea pigs
| Autor: | B.A. Chua, I. Vonder Muhll, W. Blair, P. M. Kindler, A. M. Perks, T. Ruiz |
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| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Physiology Sodium Guinea Pigs chemistry.chemical_element Iodoacetates In Vitro Techniques Sodium Channels 2 4-Dinitrophenol Absorption Guinea pig Amiloride chemistry.chemical_compound Fetus Pregnancy Physiology (medical) Internal medicine medicine Animals Respiratory system Lung Pharmacology Reabsorption Uncoupling Agents General Medicine Metabolism Iodoacetic Acid Endocrinology chemistry Extravascular Lung Water Lactates Female Glycolysis Dinitrophenols medicine.drug |
| Zdroj: | Canadian journal of physiology and pharmacology. 71(1) |
| ISSN: | 0008-4212 |
| Popis: | Lungs from near-term fetal guinea pigs (62 ± 2 days of gestation) were supported in vitro for 3 h, and lung liquid production was measured by a dye dilution technique. Twelve untreated preparations produced fluid at 1.54 ± 0.29 mL∙kg−1 body weight∙h−1 during the 1st h, with no significant changes in later hours. Twelve preparations treated with 2 × 10−4 M 2,4-dinitrophenol (DNP) showed strong reabsorptions (significant, ANOVA and regression analysis); total loss of lactate from the preparations doubled (significant, same tests). In 12 additional preparations, increasing DNP fivefold did not abolish reabsorption; results resembled those at the lower concentration. Amiloride at 10−6 M abolished reabsorptions after 2 × 10−4 M DNP, although fluid production still halted (n = 6; reductions significant, same tests). Amiloride alone had no effect (n = 6); untreated controls showed no change (n = 6). Similarly, 10−4 M sodium iodoacetate virtually abolished reabsorptions after 2 × 10−4 M DNP, although fluid production still stopped (n = 6; reductions significant, same tests). Iodoacetate alone only reduced fluid production (n = 6; significant, same tests); untreated controls showed no change (n = 6). The results suggest that reabsorptions seen after inhibition of oxidative processes depend on amiloride-sensitive Na+ channels and glycolytic metabolism.Key words: fetus, lung liquid, 2,4-dinitrophenol, amiloride, iodoacetate. |
| Databáze: | OpenAIRE |
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