BD-2 and BD-3 Increase Skin Flap Survival in a Model of Ischemia and Pseudomonas Aeruginosa Infection
| Autor: | Diogo Casal, Mário Ferraz-Oliveira, José S. Ramalho, Valentina Vassilenko, José Carlos Ferreira-Silva, Eduarda Mota-Silva, João Goyri-O'Neill, Carlos Pontinha, Luís Mascarenhas-Lemos, Paula A. Videira, Diogo Pais, Maria Guadalupe-Cabral, Inês Iria, Sara Alves |
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| Přispěvatelé: | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), UCIBIO - Applied Molecular Biosciences Unit, Centro de Estudos de Doenças Crónicas (CEDOC), LIBPhys-UNL |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Pathology beta-Defensins lcsh:Medicine medicine.disease_cause Surgical Flaps Surgical Flaps / adverse effects 0302 clinical medicine Ischemia Pseudomonas Infections / therapy Transduction Genetic Medicine CHLC CPR lcsh:Science Multidisciplinary Genetic Vectors / therapeutic use Graft Survival Surgical wound Skin Transplantation beta-Defensins / genetics Anti-Bacterial Agents Experimental models of disease Catheter Skin Transplantation / adverse effects Pseudomonas aeruginosa Immunohistochemistry Infection medicine.medical_specialty Genetic Vectors Context (language use) Anti-Bacterial Agents / metabolism Genetic Vectors / genetics Article 03 medical and health sciences CHLC PAT CLIN Surgical Flaps / microbiology Pseudomonas Infections / complications Humans Animals Pseudomonas Infections Pseudomonas aeruginosa / drug effects Rats Wistar General Anti-Bacterial Agents / therapeutic use Innate immune system Ischemia / complications business.industry lcsh:R Genetic Therapy beta-Defensins / therapeutic use medicine.disease Rats Disease Models Animal 030104 developmental biology lcsh:Q Bacterial infection Foreign body business 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-19 (2019) Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação instacron:RCAAP Scientific Reports Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) |
| Popis: | The main aim of this work was to study the usefulness of human β-defensins 2 (BD-2) and 3 (BD-3), which are part of the innate immune system, in the treatment of infected ischemic skin flaps. We investigated the effect of transducing rat ischemic skin flaps with lentiviral vectors encoding human BD-2, BD-3, or both BD-2 and BD-3, to increase flap survival in the context of a P. aeruginosa infection associated with a foreign body. The secondary endpoints assessed were: bacterial counts, and biofilm formation on the surface of the foreign body. A local ischemic environment was created by producing arterialized venous flaps in the left epigastric region of rats. Flaps were intentionally infected by placing underneath them two catheters with 105 CFU of P. aeruginosa before the surgical wounds were hermetically closed. Flap biopsies were performed 3 and 7 days post-operatively, and the specimens submitted to immunohistochemical analysis for BD-2 and BD-3, as well as to bacterial quantification. Subsequently, the catheter segments were analyzed with scanning electron microscopy (SEM). Flaps transduced with BD-2 and BD-3 showed expression of these defensins and presented increased flap survival. Rats transduced with BD-3 presented a net reduction in the number of P. aeruginosa on the surface of the foreign body and lesser biofilm formation. |
| Databáze: | OpenAIRE |
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