Circulating miR-122 levels in self-recovering hepatitis E patients
| Autor: | Vidya A. Arankalle, Bangari Haldipur |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
QH301-705.5 media_common.quotation_subject Fulminant Pathogenesis RM1-950 medicine.disease_cause Gastroenterology Hepatitis E virus Pregnancy Internal medicine medicine MiR-122 Biology (General) miRNA Subclinical infection media_common business.industry Convalescence Hepatitis E medicine.disease Therapeutics. Pharmacology business |
| Zdroj: | ExRNA, Vol 1, Iss 1, Pp 1-9 (2019) |
| ISSN: | 2398-0060 |
| DOI: | 10.1186/s41544-019-0006-0 |
| Popis: | Background Hepatitis E (HE) is prevalent in developing countries in both epidemic and sporadic forms and is characterized by high mortality during pregnancy. miR-122, the major hepatic microRNA has been shown to be modulated during liver diseases. Lack of data in HE led to investigations in non-pregnant (NPR) and pregnant (PR) patients. Results Self-recovering NPR patients and pregnant women presenting with clinical (PR-acute) or subclinical (PR-SC) HE and respective healthy controls were studied. Serum samples were tested for miR-122 levels using qRT-PCR. Acute-phase, NPR patients circulated lower miR-122 levels, reducing further during convalescence. In contrast to previous reports, circulating miR-122 levels did not correlate with serum aminotransferase (ALT). In PR-acute patients, miR-122 levels were significantly lower that reflected pregnancy status and not HEV effect. In PR-SC patients, miR-122 levels were lower than the pregnant controls and reduced further when examined one month apart. A pregnant, fulminant HE patient circulated very high miR-122 levels that increased further during convalescence. Correlation analysis of miR-122 and circulating cytokines showed moderate correlation with CCL2 (subclinical, pregnant) and IL-6 (NPR). This first report revealed downregulation of circulating miR-122 levels in self-recovering NPR-acute patients despite liver damage (raised serum ALT) suggestive of alternate mechanism of secretion in blood. Conclusion HEV infection during pregnancy led to differential modulation of serum miR-122 levels that correlated with clinical presentation. Utility of miR-122 as a prognostic marker for severe disease during pregnancy needs to be evaluated. |
| Databáze: | OpenAIRE |
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