Generation and characterization of infectious chimeric clones between human immunodeficiency virus type 1 and simian immunodeficiency virus from an African green monkey
| Autor: | Riri Shibata, Hiroyuki Sakai, A Ishimoto, T Kiyomasu, Masanori Hayami, Akio Adachi |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
viruses
Immunology Gene Products gag HIV Envelope Protein gp120 Biology Transfection Recombinant virus medicine.disease_cause Microbiology Cercopithecus aethiops Virus Cell Line Viral Envelope Proteins Virology Chlorocebus aethiops medicine Animals Humans Cloning Molecular Chimera RNA-Directed DNA Polymerase Simian immunodeficiency virus Reverse transcriptase Kinetics Cell culture Insect Science CD4 Antigens Colonic Neoplasms DNA Viral HIV-1 Simian Immunodeficiency Virus African Green Monkey Research Article |
| Zdroj: | Journal of Virology. 64:5861-5868 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.64.12.5861-5868.1990 |
| Popis: | A series of chimeric clones of human immunodeficiency virus type 1 and simian immunodeficiency virus isolated from an African green monkey was constructed in vitro. In transient transfection experiments, all clones produced virion-associated reverse transcriptase, gag proteins, and env proteins. Eight out of 10 chimeric viruses clearly grew in the human CD4+ cell line C8166. Susceptibility of other CD4+ cell lines, MT-4, A3.01, and Molt4 clone 8, to infection with these viruses was also demonstrated. |
| Databáze: | OpenAIRE |
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