Interleukin-6 Affects Aging-Related Changes of the PPARα-PGC-1α Axis in the Myocardium
| Autor: | Ewa Waszkiewicz, Karol Kamiński, Maria M. Winnicka, Tomasz A. Bonda, Magdalena Dziemidowicz, Beata Szynaka, Magdalena Sokołowska |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Aging SIRT3 Immunology Peroxisome proliferator-activated receptor 030204 cardiovascular system & hematology Mitochondrion 03 medical and health sciences Mice 0302 clinical medicine Downregulation and upregulation Virology Internal medicine Coactivator medicine Animals PPAR alpha Interleukin 6 Receptor chemistry.chemical_classification Mice Knockout biology Interleukin-6 Myocardium Cell Biology Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Mitochondria Blot Mice Inbred C57BL 030104 developmental biology Endocrinology chemistry biology.protein |
| Zdroj: | Journal of interferoncytokine research : the official journal of the International Society for Interferon and Cytokine Research. 37(12) |
| ISSN: | 1557-7465 |
| Popis: | Aging is related to gradual increase of interleukin 6 (IL-6) plasma level that affects peroxisome proliferator-activated receptor (PPAR) expression. We evaluated age-related changes in cardiac expression of PPARα, its coactivator PGC-1α, and selected downstream proteins in mice with systemic IL-6 knockout (IL6KO). Male C57BL6/J wild-type (WT) and IL6KO mice were used at the age of 16-20 weeks (young) and 24-30 months (senescent). Echocardiography and electron microscopy were applied to assess the function and ultrastructure of the heart. Western blotting and quantitative real-time PCR were used to estimate protein and mRNA levels of selected genes. PPARα expression in the myocardium of young IL6KO animals is lower and remains unchanged with aging, whereas in WT mice it declines with age and in both senescent groups it is equal. We observed aging-related upregulation of PGC-1α and less pronounced decline of Sirt3 in IL6KO animals; the level of cytochrome C was significantly decreased in IL6KO group only, suggesting disturbed mitochondrial function, which was not sufficient to evoke obvious changes in cardiac performance and function assessed by echocardiography. IL-6 and aging are involved in regulation of PPARα and PGC-1α expression and may influence the mitochondrial function. |
| Databáze: | OpenAIRE |
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