Seroprevalence of Sarcocystis falcatula in Two Islands of Malaysia using Recombinant Surface Antigen 4
| Autor: | Tengku-Idris Tengku Idzzan Nadzirah, Lau Yee Ling, Fong Mun Yik |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Veterinary medicine
Antigenicity Sarcocystosis Blotting Western 030231 tropical medicine Antigens Protozoan Enzyme-Linked Immunosorbent Assay Biology law.invention 03 medical and health sciences 0302 clinical medicine Antigen Western blot Seroepidemiologic Studies law Sarcocystis falcatula medicine Humans Parasite hosting Seroprevalence serodiagnosis 0303 health sciences medicine.diagnostic_test 030306 microbiology Malaysia Sarcocystis surface antigen biology.organism_classification Recombinant Proteins Infectious Diseases Antigens Surface Recombinant DNA Protozoa Original Article Parasitology |
| Zdroj: | The Korean Journal of Parasitology |
| ISSN: | 1738-0006 0023-4001 |
| DOI: | 10.3347/kjp.2020.58.1.1 |
| Popis: | Sarcocystosis was diagnosed worldwide by serodiagnostic tests utilising the whole parasite, for which the protozoa were maintained in vitro are more costly. In this study, antigenicity of Sarcocystis falcatula recombinant protein (rSfSAG4) was investigated towards the local communities of Pangkor and Tioman Islands and its seroprevalence was surveyed in these islands. A total of 348 human sera were tested using rSfSAG4 by Western blot and ELISA. High prevalence of sarcocystosis was observed in Tioman Island (80.6%) than in Pangkor Island (50.0%) by Western blot. In ELISA, the seroprevalence observed in Tioman Island was 45.9%, whereas in Pangkor Island 63.0%. In other parasitic infections, the prevalence was 34.0% by Western blot and 46.0% by ELISA. In healthy control group, 7% by Western blot and 8% by ELISA showed positivity to rSfSAG4. It is suggested SfSAG4 is a candidate antigen to measure seroprevalence of sarcocystosis. |
| Databáze: | OpenAIRE |
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