Exercise Training Prevents Dexamethasone-induced Rarefaction
Autor: | Naiara A. Herrera, Isley Jesus, Carlos Ferreira dos Santos, Evandro Jose Dionisio, Thiago José Dionísio, Sandra Lia do Amaral |
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Rok vydání: | 2017 |
Předmět: |
Male
0301 basic medicine medicine.medical_specialty Angiogenesis Microvascular Rarefaction Anti-Inflammatory Agents 030204 cardiovascular system & hematology Antioxidants Dexamethasone Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Physical Conditioning Animal Internal medicine medicine Animals Rats Wistar Muscle Skeletal Pharmacology biology Skeletal muscle Rats Vascular endothelial growth factor 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry Catalase Apoptosis biology.protein Cardiology and Cardiovascular Medicine medicine.drug |
Zdroj: | Journal of Cardiovascular Pharmacology. 70:194-201 |
ISSN: | 0160-2446 |
DOI: | 10.1097/fjc.0000000000000505 |
Popis: | Dexamethasone (DEX) causes rarefaction. In contrast, training (T) prevents rarefaction and stimulates angiogenesis. This study investigated the mechanisms responsible for the preventive role of T in DEX-induced rarefaction. Rats underwent T or were kept sedentary (8 weeks) and were treated with DEX or saline during the following 14 days. Tibialis anterior muscle was used for measurements of capillary density (CD), capillary-to-fiber ratio (C:F ratio), superoxide dismutase CuZn (SOD-1), superoxide dismutase MnSOD (SOD-2), catalase (CAT) mRNA as well as SOD-1, SOD-2, CAT, vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor-2 (VEGF-R2), cyclooxygenase-2 (COX-2), B-cell lymphoma 2 (Bcl-2), Bd-2-like protein 4 (Bax), p-Bax, and caspase-3 cleaved protein levels. DEX decreased CD (-38.1%), C:F ratio (-30.0%), VEGF (-19.0%), VEGFR-2 (-20.1%), COX-2 (-22.8%), Bcl-2 (-20.5%), Bcl-2/Bax ratio (-13.7%), p-Bax/Bax (-20.0%) and increased SOD-2 (+41.6%) and caspase-3 cleaved (+24.1%). Conversely, T prevented reductions in CD (+54.2%), C:F ratio (+32.9%), VEGF (+25.3%), VEGFR-2 (+22.2%), COX-2 (+31.5%), Bcl-2 (+35.5%), Bcl-2/Bax ratio (+19.9%), p-Bax/Bax (+32.1%), and caspase-3 cleaved increase (-7.8%). T increased CAT mRNA (+21.5%) in the DEX-treated group. In conclusion, T prevented the DEX-induced rarefaction by increasing antioxidant enzymes resulting in a better balance between apoptotic and anti-apoptotic protein levels. |
Databáze: | OpenAIRE |
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