Biochemical characterization of campylobacter jejuni pnpase, an exoribonuclease important for bacterial pathogenicity
| Autor: | Cecília M. Arraiano, Nabila Haddad, Rute G. Matos, Jorge Casinhas |
|---|---|
| Přispěvatelé: | Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), UMR 1014 SECurité des ALIments et Microbiologie, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Models Molecular Cations Divalent [SDV]Life Sciences [q-bio] 030106 microbiology Purine nucleoside phosphorylase Virulence Biochemistry Campylobacter jejuni 03 medical and health sciences rna metabolism ribonucleases Exoribonuclease Polynucleotide phosphorylase Ribonuclease Protein Structure Quaternary foodborne PNPase Microbial Viability biology Temperature RNA campylobacter General Medicine Biochemical Activity biology.organism_classification Cell biology RNA Bacterial Exoribonucleases biology.protein pathogen |
| Zdroj: | Biochimie Biochimie, Elsevier, 2018, 147, pp.70-79. ⟨10.1016/j.biochi.2018.01.001⟩ |
| ISSN: | 0300-9084 |
| Popis: | International audience; Bacteria need to promptly respond to environmental changes. Ribonucleases (RNases) are key factors in the adaptation to new environments by enabling a rapid adjustment in RNA levels. The exoribonuclease polynucleotide phosphorylase (PNPase) is essential for low-temperature cell survival, affects the synthesis of proteins involved in virulence and has an important role in swimming, cell adhesion/invasion ability, and chick colonization in C. jejuni. However, the mechanism of action of this ribonuclease is not yet known. In this work we have characterized the biochemical activity of C. jejuni PNPase. Our results demonstrate that Cj-PNP is a processive 3′ to 5′ exoribonuclease that degrades single-stranded RNAs. Its activity is regulated according to the temperature and divalent ions. We have also shown that the KH and S1 domains are important for trimerization, RNA binding, and, consequently, for the activity of Cj-PNP. These findings will be helpful to develop new strategies for fighting against C. jejuni and may be extrapolated to other foodborne pathogens. |
| Databáze: | OpenAIRE |
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