The role of monocytes and T cells in 1,25-dihydroxyvitamin D3 mediated inhibition of B cell function in vitro
| Autor: | Lars Kærgaard Poulsen, Torben Barington, Klaus Müller, Carsten Heilmann, Klaus Bendtzen |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment T cell T-Lymphocytes Cell Communication Biology In Vitro Techniques Lymphocyte Activation Monocytes Calcitriol Internal medicine medicine Humans B cell Pharmacology B-Lymphocytes Tumor Necrosis Factor-alpha Monocyte Lymphokine Interleukin medicine.anatomical_structure Endocrinology Cytokine Cell culture Antibody Formation Tumor necrosis factor alpha Interleukin-1 |
| Zdroj: | Müller, K, Heilmann, C, Poulsen, L K, Barington, T & Bendtzen, K 1991, ' The role of monocytes and T cells in 1,25-dihydroxyvitamin D3 mediated inhibition of B cell function in vitro ', Immunopharmacology, vol. 21, no. 2, pp. 121-8 . University of Southern Denmark University of Copenhagen |
| ISSN: | 0162-3109 |
| Popis: | Udgivelsesdato: null-null 1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3) inhibits immunoglobulin production by human mononuclear cells (MNC) in vitro. The present study was undertaken to evaluate the role of T cells and monocytes in 1,25-(OH)2D3 induced suppression of B cell functions. The synthetic vitamin D3 analogue MC 903 was examined in parallel. 1,25-(OH)2D3 and MC 903 showed a dose-related inhibition of IgM, IgG and IgA plaque-forming cells in poke-weed mitogen (PWM) activated cultures of MNC. This effect was most likely mediated through impairment of T cell and monocyte functions. First, the inhibitory effect was seen after PWM stimulation, but not after Epstein-Barr virus stimulation which activates B cells independently of T cells and monocytes. Second, 1,25-(OH)2D3 was not effective in T cell and monocyte-depleted cultures. Third, the effect of 1,25-(OH)2D3 on PWM driven MNC was reversed by addition of the recombinant monokines: interleukin (IL)-1 beta, tumour necrosis factor alpha (rTNF alpha), rIL-6, as well as the lymphokines: lymphotoxin (rLT) and rIL-2. This is consistent with the finding that 1,25-(OH)2D3 also inhibited IL-1 alpha, TNF alpha and LT production in these cultures. The assumption that B cells are not directly affected by 1,25-(OH)2D3 was further supported by the fact that 24 h of culture with 10(-8) M 1,25-(OH)2D3 failed to reduce immunoglobulin production by in vivo activated B cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|