Conditional destabilization of the TPLATE complex impairs endocytic internalization
| Autor: | Qihang Jiang, Klaas Yperman, Daniël Van Damme, Bert De Rybel, Dominique Eeckhout, Peter Grones, Geert De Jaeger, Eliana Mor, Jonah Nolf, Evelien Mylle, Jonathan Dragwidge, Jie Wang, Roman Pleskot |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
0106 biological sciences
0303 health sciences Programmed cell death Multidisciplinary Chemistry Arabidopsis Proteins Protein subunit media_common.quotation_subject Endocytic cycle Autophagy Arabidopsis Receptor-mediated endocytosis Biological Sciences Endocytosis 01 natural sciences Cell biology 03 medical and health sciences Cytoplasm Mutation Internalization Heat-Shock Response 030304 developmental biology 010606 plant biology & botany media_common |
| Zdroj: | Proc Natl Acad Sci U S A Proceedings of the National Academy of Sciences |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.2023456118 |
| Popis: | In plants, endocytosis is essential for many developmental and physiological processes, including regulation of growth and development, hormone perception, nutrient uptake, and defense against pathogens. Our toolbox to modulate this process is, however, rather limited. Here, we report a conditional tool to impair endocytosis. We generated a partially functional TPLATE allele by substituting the most conserved domain of the TPLATE subunit of the endocytic TPLATE complex (TPC). This substitution destabilizes TPC and dampens the efficiency of endocytosis. Short-term heat treatment increases TPC destabilization and reversibly delocalizes TPLATE from the plasma membrane to aggregates in the cytoplasm. This blocks FM uptake and causes accumulation of various known endocytic cargoes at the plasma membrane. Short-term heat treatment therefore transforms the partially functional TPLATE allele into an effective conditional tool to impair endocytosis. Next to their role in endocytosis, several TPC subunits are also implicated in actin-regulated autophagosomal degradation. Inactivating TPC via the WDX mutation, however, does not impair autophagy, thus enabling specific and reversible modulation of endocytosis in planta. |
| Databáze: | OpenAIRE |
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