Chorda-evoked opening of tight junctions in rat submandibular salivary acini demonstrated by microperoxidase
| Autor: | Noriyasu Takai, Yo Yoshida, Hirotaka Murakami, Haruki Miyao, Kenji Uchihashi |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Osmosis Sucrose medicine.medical_specialty Water flow Hypertonic Solutions Submandibular Gland Lumen (anatomy) Permeability Tight Junctions Body Water stomatognathic system Internal medicine medicine Animals Rats Wistar General Dentistry Secretory pathway Tight junction Chemistry Cell Biology General Medicine Submandibular gland Electric Stimulation Rats Microscopy Electron Endocrinology medicine.anatomical_structure Peroxidases Otorhinolaryngology Paracellular transport Ultrastructure Biophysics Tonicity Indicators and Reagents Chorda Tympani Nerve |
| Zdroj: | Archives of Oral Biology. 40:1077-1080 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/0003-9969(95)00064-v |
| Popis: | The permeability of these junctions from the interstitium to the lumen was examined by using an ultrastructural tracer, microperoxidase, in conjunction with electron microscopy. In the resting gland, the reaction product of microperoxidase was seen in the interstitial and intercellular spaces, but not within acinar lumina; thus the tight junction was impermeable to microperoxidase (junction closed). Intraductal injection of hypertonic sucrose solution (1000 mOsm; 30 microliters) caused a sustained elevation of the luminal pressure, indicating osmotic water flow into the lumen due to the presence of a hypertonic solution. In this gland no opening of the tight junctions was observed. In the chorda-stimulated gland, microperoxidase entered the lumen through the tight junctions, that is, they became permeable to microperoxidase (junction open). These findings suggest that chorda stimulation opens the acinar tight junctions and that the paracellular secretory pathway may be involved in the secretion of small molecules and water from the submandibular acini. |
| Databáze: | OpenAIRE |
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