| Autor: |
Isa Palacios, Lucy S. Williams, Raymond E. Goldstein, Sujoy Ganguly |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Biophysical Journal. 102(3) |
| ISSN: |
0006-3495 |
| DOI: |
10.1016/j.bpj.2011.11.3784 |
| Popis: |
The persistent circulation of the cytoplasm, called cytoplasmic streaming, occurs in a variety of eukaryotic cells. One context in which streaming occurs is during the establishment of Drosophila body axes, when Kinesin-1 transports the axes determinants and drives cytoplasmic streaming. Although Kinesin is essential for flows, neither the mechanism by which Kinesin induces streaming nor the impact of these flows on transport are known. We have succeeded in a precise quantitative measurement of the statistical properties of streaming by Particle Image Velocimetry. We have combined these measurements with an in vivo study of the cytoplasm rheology, to calculate the energy dissipation due to streaming. Since Kinesin is required for flows we can relate the energy dissipated to the work done on the fluid by Kinesin and determine the minimum number of motors necessary to drive streaming. Furthermore we have performed these measurements on mutants that effect Kinesin-1 motor activity and found remarkable agreement between our in vivo measurements and in vitro studies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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