Developmental roles of Auxin Binding Protein 1 in Arabidopsis thaliana

Autor: Markéta Pernisová, Zlata Pavlovičová, Zuzana Gelová, Matouš Glanc, Teva Vernoux, Jaroslav Michalko, Marta Zwiewka, Tongda Xu, Michelle Gallei, Jiří Friml, Géraldine Brunoud, Lukas Hoermayer, Inge Verstraeten, Jakub Hajný, Matyáš Fendrych, Xixi Zhang, Milada Čovanová, Robert Hauschild, Lanxin Li, Huibin Han
Přispěvatelé: Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), European Research Council (ERC)742985Austrian Science Fund (FWF)I 3630-B25DOC Fellowship of the Austrian Academy of SciencesEuropean Structural and Investment Funds, Operational Programme Research, Development and EducationCZ.02.2.69/0.0/0.0/17_050/0008496Grant Agency of the Czech RepublicGA 20-20860YMEYS CRCZ.02.1.01/0.0/0.0/16_019/0000738, École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0106 biological sciences
0301 basic medicine
Mutant
Regulator
Arabidopsis
Receptors
Cell Surface
Plant Science
Biology
Real-Time Polymerase Chain Reaction
01 natural sciences
Plant Roots
03 medical and health sciences
Plant Growth Regulators
Auxin
Gene Expression Regulation
Plant
Genetics
Arabidopsis thaliana
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
[SDV.BDD]Life Sciences [q-bio]/Development Biology
Plant Proteins
chemistry.chemical_classification
Bolting
Microscopy
Confocal
Indoleacetic Acids
Arabidopsis Proteins
Protoplasts
Lateral root
fungi
AUXIN BINDING PROTEIN 1 (ABP1)AuxinPlant developmentAuxin signaling
food and beverages
General Medicine
biology.organism_classification
Hypocotyl
Cell biology
030104 developmental biology
chemistry
Agronomy and Crop Science
Intracellular
Function (biology)
010606 plant biology & botany
Zdroj: Plant Science
Plant Science, 2021, 303, pp.110750. ⟨10.1016/j.plantsci.2020.110750⟩
Plant Science, Elsevier, 2021, 303, pp.110750. ⟨10.1016/j.plantsci.2020.110750⟩
ISSN: 0168-9452
Popis: International audience; Auxin is a major plant growth regulator, but current models on auxin perception and signaling cannot explain the whole plethora of auxin effects, in particular those associated with rapid responses. A possible candidate for a component of additional auxin perception mechanisms is the AUXIN BINDING PROTEIN 1 (ABP1), whose function in planta remains unclear. Here we combined expression analysis with gain-and loss-of-function approaches to analyze the role of ABP1 in plant development. ABP1 shows a broad expression largely overlapping with, but not regulated by, transcriptional auxin response activity. Furthermore, ABP1 activity is not essential for the transcriptional auxin signaling. Genetic in planta analysis revealed that abp1 loss-of-function mutants show largely normal development with minor defects in bolting. On the other hand, ABP1 gain-of-function alleles show a broad range of growth and developmental defects, including root and hypocotyl growth and bending, lateral root and leaf development, bolting, as well as response to heat stress. At the cellular level, ABP1 gain-of-function leads to impaired auxin effect on PIN polar distribution and affects BFA-sensitive PIN intracellular aggregation. The gain-of-function analysis suggests a broad, but still mechanistically unclear involvement of ABP1 in plant development, possibly masked in abp1 loss-of-function mutants by a functional redundancy.
Databáze: OpenAIRE
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