Developmental roles of Auxin Binding Protein 1 in Arabidopsis thaliana
Autor: | Markéta Pernisová, Zlata Pavlovičová, Zuzana Gelová, Matouš Glanc, Teva Vernoux, Jaroslav Michalko, Marta Zwiewka, Tongda Xu, Michelle Gallei, Jiří Friml, Géraldine Brunoud, Lukas Hoermayer, Inge Verstraeten, Jakub Hajný, Matyáš Fendrych, Xixi Zhang, Milada Čovanová, Robert Hauschild, Lanxin Li, Huibin Han |
---|---|
Přispěvatelé: | Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), European Research Council (ERC)742985Austrian Science Fund (FWF)I 3630-B25DOC Fellowship of the Austrian Academy of SciencesEuropean Structural and Investment Funds, Operational Programme Research, Development and EducationCZ.02.2.69/0.0/0.0/17_050/0008496Grant Agency of the Czech RepublicGA 20-20860YMEYS CRCZ.02.1.01/0.0/0.0/16_019/0000738, École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL) |
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine Mutant Regulator Arabidopsis Receptors Cell Surface Plant Science Biology Real-Time Polymerase Chain Reaction 01 natural sciences Plant Roots 03 medical and health sciences Plant Growth Regulators Auxin Gene Expression Regulation Plant Genetics Arabidopsis thaliana [SDV.BV]Life Sciences [q-bio]/Vegetal Biology [SDV.BDD]Life Sciences [q-bio]/Development Biology Plant Proteins chemistry.chemical_classification Bolting Microscopy Confocal Indoleacetic Acids Arabidopsis Proteins Protoplasts Lateral root fungi AUXIN BINDING PROTEIN 1 (ABP1)AuxinPlant developmentAuxin signaling food and beverages General Medicine biology.organism_classification Hypocotyl Cell biology 030104 developmental biology chemistry Agronomy and Crop Science Intracellular Function (biology) 010606 plant biology & botany |
Zdroj: | Plant Science Plant Science, 2021, 303, pp.110750. ⟨10.1016/j.plantsci.2020.110750⟩ Plant Science, Elsevier, 2021, 303, pp.110750. ⟨10.1016/j.plantsci.2020.110750⟩ |
ISSN: | 0168-9452 |
Popis: | International audience; Auxin is a major plant growth regulator, but current models on auxin perception and signaling cannot explain the whole plethora of auxin effects, in particular those associated with rapid responses. A possible candidate for a component of additional auxin perception mechanisms is the AUXIN BINDING PROTEIN 1 (ABP1), whose function in planta remains unclear. Here we combined expression analysis with gain-and loss-of-function approaches to analyze the role of ABP1 in plant development. ABP1 shows a broad expression largely overlapping with, but not regulated by, transcriptional auxin response activity. Furthermore, ABP1 activity is not essential for the transcriptional auxin signaling. Genetic in planta analysis revealed that abp1 loss-of-function mutants show largely normal development with minor defects in bolting. On the other hand, ABP1 gain-of-function alleles show a broad range of growth and developmental defects, including root and hypocotyl growth and bending, lateral root and leaf development, bolting, as well as response to heat stress. At the cellular level, ABP1 gain-of-function leads to impaired auxin effect on PIN polar distribution and affects BFA-sensitive PIN intracellular aggregation. The gain-of-function analysis suggests a broad, but still mechanistically unclear involvement of ABP1 in plant development, possibly masked in abp1 loss-of-function mutants by a functional redundancy. |
Databáze: | OpenAIRE |
Externí odkaz: |