Comparison of plasma progesterone assay in women by competitive protein binding (CPB) and radioimmunoassay (RIA)
| Autor: | Emilio Mattar, Bernardo Léo Wajchenberg, Pieroni Rr, Helena Wachslicht, A. C. Lerario, Seico Hanada Sugawara, Carlos Henrique de Mesquita |
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| Rok vydání: | 1976 |
| Předmět: |
endocrine system
Clinical Biochemistry Radioimmunoassay Plasma protein binding Luteal Phase Luteal phase Binding Competitive Biochemistry Guinea pig Radioligand Assay Follicular phase Humans Bovine serum albumin Progesterone Antiserum Chromatography biology Chemistry Biochemistry (medical) General Medicine Follicular Phase Evaluation Studies as Topic biology.protein Female Chromatography Thin Layer hormones hormone substitutes and hormone antagonists |
| Zdroj: | Clinica Chimica Acta. 68:67-72 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(76)90289-8 |
| Popis: | Plasma progesterone levels during the follicular and luteal phases were compared when measured simultaneously by competitive protein binding (using guinea pig sera as binding agent) and radioimmunoassay (antiserum against progesterone-11-alpha-succinyl bovine serum albumin in rabbits), the values obtained were significantly different within each technique, depending on whether previous thin-layer chromatographic purification of the extracts was employed or not. No significant differences were noticed between CPB and RIA when the chromatographic step was used, but when it was omitted, CPB was greater than RIA at the follicular but not at the luteal phase. |
| Databáze: | OpenAIRE |
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