Co-assembly and multicomponent hydrogel formation upon mixing nucleobase-containing peptides
| Autor: | Sabine Bouguet-Bonnet, Marie-Christine Averlant-Petit, Loic Stefan, Tristan Giraud, Guillaume Pickaert, Lionel Richaudeau, Marie-José Stébé |
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| Přispěvatelé: | Laboratoire de Chimie Physique Macromoléculaire (LCPM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Cristallographie, Résonance Magnétique et Modélisations (CRM2), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut Jean Barriol (IJB), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Lorrain de Chimie Moléculaire (L2CM), ANR-20-CE06-0010,MUNCH,Hydrogels nucléopeptidiques multi-composés(2020) |
| Rok vydání: | 2021 |
| Předmět: |
Circular dichroism
Materials science Supramolecular chemistry Peptide Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Nucleobase X-Ray Diffraction Scattering Small Angle [CHIM]Chemical Sciences General Materials Science chemistry.chemical_classification Rheometry Small-angle X-ray scattering Hydrogels [CHIM.MATE]Chemical Sciences/Material chemistry Polymer 021001 nanoscience & nanotechnology Nanostructures 0104 chemical sciences chemistry Self-healing hydrogels Peptides 0210 nano-technology |
| Zdroj: | Nanoscale Nanoscale, Royal Society of Chemistry, 2021, ⟨10.1039/D1NR02417E⟩ |
| ISSN: | 2040-3372 2040-3364 |
| Popis: | International audience; Peptide-based hydrogels are physical gels formed through specific supramolecular self-assembling processes, leading to ordered nanostructures which constitutes the water entrapping scaffold of the soft material. Thanks to the inherent properties of peptides, these hydrogels are highly considered in the biomedical domain and open new horizons in terms of application in advanced therapies and biotechnologies. The use of one, and only one, native peptide to formulate a gel is by far the most reported approach to design such materials, but suffers from several limitations, including in terms of mechanical properties. To improve peptide-based hydrogels interest and give rise to innovative properties, several strategies have been proposed in the recent years, and the development of multicomponent peptide-based hydrogels appears as a promising and relevant strategy. Indeed, mixing two or more compounds to develop new materials is a much-used approach that has proven its effectiveness in a wide variety of domains, including polymers, composites and alloys. While still limited to a handful of examples, we would like to report herein on the formulation and the comprehensive study of multicomponent hybrid DNA-nucleobase/peptide-based hydrogels using a multiscale approach based on a large panel of analytical techniques (i.e., rheometry, proton relaxometry, SAXS, electronic microscopy, infrared, circular dichroism, fluorescence, Thioflavin T assays). Among the six multicomponent systems studied, the results highlight the synergistic role of the presence of the two complementary DNA-nucleobases (i.e., adenine/thymine and guanine/cytosine) on the coassembling process from structural (e.g., morphology of the nanoobjects) to physicochemical (e.g., kinetics of formation, fluorescence properties) and mechanical properties (e.g., stiffness, resistance to external stress). All the data confirm the relevance of the multicomponent peptide-based approach in the design of innovative hydrogels and bring another brick in the wall of the understanding of these complex and promising systems. |
| Databáze: | OpenAIRE |
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