The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants
Autor: | Frederic Chaux-Jukic, Sandro Keller, David Zimmer, Torsten Möhlmann, Martin Jung, Karin Gries, Felix Willmund, Marina Rohr, Vincent Leon Gotsmann, Timo Mühlhaus, Fabian Ries, Claudia Herkt, Michael Schroda, Jens Christmann, Frederik Sommer, Raphael Trösch, Lisa Désirée Westrich |
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Rok vydání: | 2019 |
Předmět: |
Models
Molecular 0106 biological sciences Physiology Population Arabidopsis Chlamydomonas reinhardtii Plant Science 01 natural sciences Genetics Protein biosynthesis Plastid education Plant Proteins education.field_of_study biology Chemistry food and beverages Articles biology.organism_classification Cell biology Chloroplast Protein Biosynthesis Chaperone (protein) biology.protein Biogenesis 010606 plant biology & botany |
Zdroj: | Plant Physiology. 179:1093-1110 |
ISSN: | 1532-2548 0032-0889 |
Popis: | Biochemical processes in chloroplasts are important for virtually all life forms. Tight regulation of protein homeostasis and the coordinated assembly of protein complexes, composed of both imported and locally synthesized subunits, are vital to plastid functionality. Protein biogenesis requires the action of cotranslationally acting molecular chaperones. One such chaperone is trigger factor (TF), which is known to cotranslationally bind most newly synthesized proteins in bacteria, thereby assisting their correct folding and maturation. However, how these processes are regulated in chloroplasts remains poorly understood. We report here functional investigation of chloroplast-localized TF (TIG1) in the green alga (Chlamydomonas reinhardtii) and the vascular land plant Arabidopsis (Arabidopsis thaliana). We show that chloroplastic TIG1 evolved as a specialized chaperone. Unlike other plastidic chaperones that are functionally interchangeable with their prokaryotic counterpart, TIG1 was not able to complement the broadly acting ortholog in Escherichia coli. Whereas general chaperone properties such as the prevention of aggregates or substrate recognition seems to be conserved between bacterial and plastidic TFs, plant TIG1s differed by associating with only a relatively small population of translating ribosomes. Furthermore, a reduction of plastidic TIG1 levels leads to deregulated protein biogenesis at the expense of increased translation, thereby disrupting the chloroplast energy household. This suggests a central role of TIG1 in protein biogenesis in the chloroplast. |
Databáze: | OpenAIRE |
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