Immunogenicity of recombinant Omp16 protein of Pasteurella multocida B:2 in mouse model

Autor:	Shivachandra, S. B., Abhinendra Kumar, Mohanty, N. N., Yogisharadhya, R.
Rok vydání:	2017
Předmět:	General Veterinary Animal Science and Zoology
Zdroj:	Scopus-Elsevier
ISSN:	2394-3327 0367-8318
DOI:	10.56093/ijans.v87i1.66834
Popis:	Bacterial peptidoglycan-associated lipoproteins (PAL) are potential targets for the development of diagnostics/subunit vaccines for infectious diseases. Most commonly prevalent Omp16 lipoprotein is absolutely conserved among Pasteurella multocida strains, which are involved in multiple infectious diseases of livestock worldwide. In the present study, we cloned omp16 gene encoding for mature Omp16 of P. multocida B:2 strain P52 and overexpressed as a fusion protein in Escherichia coli. Mice immunized with purified recombinant non-lipidated Omp16 fusion protein (~32 kDa) resulted in elicitation of significant antigen specific serum antibody titres (total IgG and subtypes). A more pronounced increase in Th2 response (IgG1) was noticed. The study indicated the potential possibilities to use lipidated recombinant Omp16 protein in developing a composite subunit vaccine along with suitable adjuvant for haemorrhagic septicaemia/ pasteurellosis in livestock.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bab6ec63b19577e8c7dff2d64a390822 https://doi.org/10.56093/ijans.v87i1.66834 Zobrazit plný text záznamu