An electrochemical method for measuring metabolic activity and counting cells

Autor:	Alexander I. Yaropolov, A. S. Kaprel’yants, M. T. Khlupova, B.A Kuznetsov, Sergey Shleev
Rok vydání:	2006
Předmět:	Detection limit Mycobacterium smegmatis fungi Analytical chemistry Biology biology.organism_classification Electrochemistry Cell counting Applied Microbiology and Biotechnology Biochemistry Electron transport chain Sample preparation Metabolic activity Bacteria
Zdroj:	Scopus-Elsevier
ISSN:	1608-3024 0003-6838
DOI:	10.1134/s0003683806050140
Popis:	An express electrochemical method for determining the metabolic activity of live cells based on the possibility of an electron exchange between an electrode and elements of the biological electron transfer chain in the presence of a mediator is proposed. This method is useful for studying any live cells (animal, plant, and microbial), including anaerobic, dormant, and spore cells. The sample preparation and measurement itself does not take more than 30 min. The detection limit in a volume of 15 ml amounts to 105 cells/ml. The applicability of the assessment method of the metabolic activity level during the transition of the bacteria Mycobacterium smegmatis into an uncultivable dormant state was demonstrated. This method is of special value for medicine and environmental control, detecting latent forms of pathogens. An optimal combination of the methods for the express analysis of latent pathogens is proposed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bab65efcffbab6ff7d797616f785a027 https://doi.org/10.1134/s0003683806050140 Zobrazit plný text záznamu Plný text ve formátu PDF