Chemical analysis of the Alphaproteobacterium strain MOLA1416 associated with the marine lichen Lichina pygmaea
| Autor: | Laurent Intertaglia, Delphine Parrot, Philippe Jéhan, Sophie Tomasi, Marcelino T. Suzuki, Martin Grube |
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| Přispěvatelé: | Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre Régional de Mesures Physiques de l'Ouest (CRMPO), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), PIERRE FABRE-EDF (EDF)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), 10-INBS-02-01, CNRS, Centre National de la Recherche Scientifique, UPMC, University of Pittsburgh Medical Center, Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Lichens Cell Survival Metabolite Isocoumarins Melanoma Experimental Plant Science Horticulture Biochemistry Cell Line Mice Structure-Activity Relationship 03 medical and health sciences chemistry.chemical_compound Metabolomics Botany Animals Humans [CHIM]Chemical Sciences Lichina pygmaea Molecular networking Molecular Biology Diketopiperazines Cell Proliferation Alphaproteobacteria Dose-Response Relationship Drug Molecular Structure biology Strain (chemistry) Prodigiosin General Medicine 16S ribosomal RNA biology.organism_classification Dereplication workflow 030104 developmental biology chemistry LC/MSn Mycoplana ramosa Lichen-associated bacteria Oligopeptides |
| Zdroj: | Phytochemistry Phytochemistry, Elsevier, 2018, 145, pp.57-67. ⟨10.1016/j.phytochem.2017.10.005⟩ Phytochemistry, 2018, 145, pp.57-67. ⟨10.1016/j.phytochem.2017.10.005⟩ |
| ISSN: | 0031-9422 |
| DOI: | 10.1016/j.phytochem.2017.10.005 |
| Popis: | International audience; Alphaproteobacterium strain MOLA1416, related to Mycoplana ramosa DSM 7292 and Chelativorans intermedius CC-MHSW-5 (93.6% 16S rRNA sequence identity) was isolated from the marine lichen, Lichina pygmaea and its chemical composition was characterized by a metabolomic network analysis using LC-MS/MS data. Twenty-five putative different compounds were revealed using a dereplication workflow based on MS/MS signatures available through GNPS (https://gnps.ucsd.edu/). In total, ten chemical families were highlighted including isocoumarins, macrolactones, erythrinan alkaloids, prodiginines, isoflavones, cyclohexane-diones, sterols, diketopiperazines, amino-acids and most likely glucocorticoids. Among those compounds, two known metabolites (13 and 26) were isolated and structurally identified and metabolite 26 showed a high cytotoxic activity against B16 melanoma cell lines with an IC50 0.6 ± 0.07 μg/mL. © 2017 Elsevier Ltd |
| Databáze: | OpenAIRE |
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