Temporal variations shape the gut microbiome ecology of the moth Brithys crini

Autor: Jesús Marín‐Miret, Francisco González‐Serrano, Tania Rosas, Joaquín Baixeras, Amparo Latorre, Ana Elena Pérez‐Cobas, Andrés Moya
Přispěvatelé: Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Generalitat Valenciana, European Commission, Comunidad de Madrid
Rok vydání: 2022
Předmět:
Zdroj: Environmental Microbiology. 24:3939-3953
ISSN: 1462-2920
1462-2912
2018-0993
DOI: 10.1111/1462-2920.15952
Popis: Most insects harbour associated microbial communities contributing to host physiology, in some cases specialized to allow them to live in recalcitrant environments. However, from insects, Lepidoptera microbiomes' stability and metabolism are poorly understood. One example is the noctuid moth Brithys crini that feeds on a plant rich in alkaloids, and neither the associated microbial communities dynamics over time nor role in secondary metabolism has been deeply explored. Here, through 16S rRNA gene high-throughput sequencing, combined with functional prediction, we characterized in-depth the midgut and hindgut bacterial composition of this species larvae by sampling it over 3 years (n = 114). The gut-microbiota in this insect is significantly shaped by temporal and environmental changes affecting the host-plant dynamics over time. Specifically, different time scales (year, season, and collection date) and associated climatic variables as temperature or precipitation strongly influence the diversity, composition and metabolic capabilities of B. crini’s gut microbial communities. Despite the physicochemical difference of the midgut and hindgut in Lepidoptera, no variations in diversity and functions were found pointing to a generalist capacity of the gut microbiota or a constant microbial acquirement from the host plant environment. We also found microbial candidate genes potentially involved in plant environment detoxification through a predictive functional approach that needs further investigation for bioremediation and ecological applications.
This work was supported by grants to A.L. from the Spanish Ministry of Science and Innovation (PGC2018-099344-B-I00), and to A.M. from Generalitat Valenciana (project Prometeo/2018/133), and co-financed by the European Regional Development Fund (ERDF). A.E.P.-C. is recipient of a Grant for the Attraction of Talent within the Comunidad de Madrid (grant number 2019-T2/BMD-12874).
Databáze: OpenAIRE
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