DNA loop domain organization in nucleoids from cells of different types
Autor: | L. L. Lukash, Marianna Chopei, Anastasia Semenova, Andrei Sivolob, Alexandra Lozovik, Katerina Afanasieva |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Adult Male Cell type animal structures Cell Biophysics Biology Biochemistry 03 medical and health sciences chemistry.chemical_compound Transcriptional regulation medicine Nucleoid Humans Lymphocytes Molecular Biology Gel electrophoresis Cell Biology DNA Molecular biology Cell Nucleus Structures Chromatin Comet assay Kinetics 030104 developmental biology medicine.anatomical_structure chemistry bacteria Female Comet Assay |
Zdroj: | Biochemical and biophysical research communications. 483(1) |
ISSN: | 1090-2104 |
Popis: | The loop domain organization of chromatin plays an important role in transcription regulation and thus may be assumed to vary in cells of different types. We investigated the kinetics of DNA loop migration during single cell gel electrophoresis (the comet assay) for nucleoids obtained from human lymphocytes, lymphoblasts and glioblastoma T98G cells. The results confirm our previous observation that there are three parts of DNA in nucleoids: DNA on the nucleoid surface, loops up to ∼150 kb inside the nucleoid, and larger loops that cannot migrate. However, the relative amounts of the three parts were found to be very different for different cell types. The distributions of the loop length up to 150 kb were shown to be exponential, with the distribution parameter, the loop density, to be dependent on the cell type. |
Databáze: | OpenAIRE |
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