Biochemical and molecular characterization of a mutation that confers a decreased raffinosaccharide and phytic acid phenotype on soybean seeds
| Autor: | William D. Hitz, Thomas J. Carlson, Scott Anthony Sebastian, Phil S. Kerr |
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| Rok vydání: | 2002 |
| Předmět: |
Sucrose
Phytic Acid Physiology Lysine Mutant Oligosaccharides Plant Science Biology Disaccharides Phosphates chemistry.chemical_compound Raffinose Genetics Asparagine Amino Acid Sequence Phytic acid Myo-Inositol-1-Phosphate Synthase Sequence Homology Amino Acid food and beverages Metabolism Phosphate Galactosyltransferases Phenotype Biochemistry chemistry Glycine Mutation Seeds sense organs Soybeans Inositol Research Article |
| Zdroj: | Plant physiology. 128(2) |
| ISSN: | 0032-0889 |
| Popis: | A single, recessive mutation in soybean (Glycine max L. Merr.), which confers a seed phenotype of increased inorganic phosphate, decreased phytic acid, and a decrease in total raffinosaccharides, has been previously disclosed (S.A. Sebastian, P.S. Kerr, R.W. Pearlstein, W.D. Hitz [2000] Soy in Animal Nutrition, pp 56–74). The genetic lesion causing the multiple changes in seed phenotype is a single base change in the third base of the codon for what is amino acid residue 396 of the mature peptide encoding a seed-expressed myo-inositol 1-phospate synthase gene. The base change causes residue 396 to change from lysine to asparagine. That amino acid change decreases the specific activity of the seed-expressed myo-inositol 1-phosphate synthase by about 90%. Radio tracer experiments indicate that the supply ofmyo-inositol to the reaction, which converts UDP-galactose and myo-inositol to galactinol is a controlling factor in the conversion of total carbohydrate into the raffinosaccharides in both wild-type and mutant lines. That same decrease in myo-inositol 1-phosphate synthetic capacity leads to a decreased capacity for the synthesis ofmyo-inositol hexaphosphate (phytic acid) and a concomitant increase in inorganic phosphate. |
| Databáze: | OpenAIRE |
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