Effect of endocervical specimen quality on detection of Chlamydia trachomatis and on the incidence of false-positive results with the Chlamydiazyme method
| Autor: | C L Murray, J S Levisky, James A. Kellogg, J W Seiple |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Adult
Microbiology (medical) Pathology medicine.medical_specialty Adolescent Papanicolaou stain Chlamydia trachomatis Biology medicine.disease_cause Antigen Predictive Value of Tests medicine Humans False Positive Reactions Chlamydiaceae Child Aged Vaginal Smears Antigens Bacterial Bacteriological Techniques Incidence Syringes Papanicolaou Test Chlamydia Infections Middle Aged biology.organism_classification Staining Chlamydiales Monoclonal Cervix Mucus Female Research Article |
| Zdroj: | Journal of Clinical Microbiology. 28:1108-1113 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.28.6.1108-1113.1990 |
| Popis: | Duplicate endocervical swabs were collected from 1,675 patients to assess the effects of variations in specimen quality on Chlamydiazyme (Abbott Laboratories) detection of Chlamydia trachomatis and the incidence of false-positive results. One swab (at random) from each patient was tested for C. trachomatis antigen by using the standard Chlamydiazyme procedure. A 200-microliter volume of 0.9% saline was added to the other swab from each patient. After vortexing, 20 microliters was smeared on a slide for Papanicolaou (Pap) staining and the remaining specimen was then tested with the Chlamydiazyme assay. The Chlamydiazyme result was positive for 170 (10.1%) and 165 (9.8%) of the stained and unstained duplicate specimens, respectively (no significant difference). Pap stains on smears from 1,536 (91.7%) of the patients were analyzed, and endocervical and/or metaplastic (E-M) cells were detected in 789 (51.4%) smears. Of these 1,536 stained and analyzed specimens, 150 (9.8%) were Chlamydiazyme positive but only 132 (88.0%) of the positive results were confirmed by repeating the test and using a monoclonal blocking antibody (Abbott). Confirmed Chlamydiazyme-positive results were obtained from only 34 (4.6%) of 747 specimens lacking E-M cells but from 98 (12.4%) of 789 specimens containing the cells (P less than 0.001). Of the 150 initially Chlamydiazyme-positive results obtained with Pap-stained, analyzed specimens, 12 (26.1%) of 46 were falsely positive from specimens lacking E-M cells but only 6 (5.8%) of 104 were falsely positive from specimens containing E-M cells (P less than 0.01). C. trachomatis antigen was detected significantly more frequently and false-positive results were significantly less common from specimens in which E-M cells were detected. |
| Databáze: | OpenAIRE |
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