Artificial nanozyme based on platinum nanoparticles anchored metal-organic frameworks with enhanced electrocatalytic activity for detection of telomeres activity
| Autor: | Caihua Qian, Jinjin Yu, Feng Gao, Pinghua Ling |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Biomedical Engineering
Biophysics Metal Nanoparticles 02 engineering and technology Biosensing Techniques Electrochemistry Platinum nanoparticles 01 natural sciences Catalysis chemistry.chemical_compound Limit of Detection Humans Telomerase Metal-Organic Frameworks Platinum Exonuclease III biology Chemistry Artificial enzyme fungi 010401 analytical chemistry General Medicine Electrochemical Techniques Hydrogen Peroxide Telomere 021001 nanoscience & nanotechnology Combinatorial chemistry 0104 chemical sciences Electrochemical gas sensor biology.protein Metal-organic framework 0210 nano-technology DNA Biotechnology HeLa Cells |
| Zdroj: | Biosensorsbioelectronics. 149 |
| ISSN: | 1873-4235 |
| Popis: | This study reports a new artificial nanozyme based on ultra-small Pt nanoparticles (Pt NPs) grown on nanoscale metalloporphyrin metal organic frameworks (P-MOF(Fe)) (termed as Pt@P-MOF(Fe)) as biomimetic catalysts and redox mediator to detect the telomerase activity. In this system, the P-MOF(Fe) were used as nanocarrier and signal media. The DNA functionalized Pt@P-MOF(Fe) was as signal probe and exhibited enhanced electrochemical signal in the presence of H2O2, owing to the synergistic effect between P-MOF(Fe) and Pt NPs. Upon the addition cell extract, the telomerase primer could extend and then hybridize with assistant DNA2 in the triple-helix, leading to the structure of triple-helix changes and release the hairpin DNA to hybridize with the capture DNA on the surface of Pt@P-MOF(Fe), resulting in the electrochemical signal readout of H2O2 reduction. With the aid of recycling amplification of Exonuclease III, the telomeres sensor exhibited the detection down to 20 Hela cell mL-1. This work supplies a new avenue to design artificial enzyme catalysts and serves as an ideal platform to use metalloporphyrin metal organic frameworks as signal media for detection of analytes. |
| Databáze: | OpenAIRE |
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