Defence response of tomato seedlings to oxidative stress induced by phenolic compounds from dry olive mill residue
| Autor: | Inmaculada García-Romera, Mercedes García-Sánchez, Ilda Casimiro, Elisabet Aranda, P. J. Casero, Francisco Espinosa, Inmaculada Garrido |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Environmental Engineering
Antioxidant Health Toxicology and Mutagenesis medicine.medical_treatment Glutathione reductase Germination medicine.disease_cause Waste Disposal Fluid Superoxide dismutase chemistry.chemical_compound Solanum lycopersicum Phenols Malondialdehyde Olea medicine Environmental Chemistry Food science Trametes versicolor Singlet Oxygen biology Fungi Public Health Environmental and Occupational Health food and beverages Hydrogen Peroxide General Medicine General Chemistry Pycnoporus cinnabarinus biology.organism_classification Pollution Enzymes Oxidative Stress Biodegradation Environmental Biochemistry chemistry Seedlings Catalase biology.protein Oxidative stress |
| Zdroj: | Chemosphere. 89:708-716 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2012.06.026 |
| Popis: | ADOR is an aqueous extract obtained from the dry olive mill residue (DOR) which contains the majority of its soluble phenolic compounds, which are responsible for its phytotoxic properties. Some studies have shown that ADOR negatively affects seed germination. However, to date, few studies have been carried out on the effect of ADOR on the oxidative stress of the plant. It is well known that saprobe fungi can detoxify these phenolic compounds and reduce the potential negative effects of ADOR on plants. To gain a better understanding of the phytotoxic effects and oxidative stress caused by this residue, tomato seeds were germinated in the presence of ADOR, treated and untreated with Coriolopsis rigida, Trametes versicolor, Pycnoporus cinnabarinus and Penicillium chrysogenum-10 saprobe fungi. ADOR sharply reduced tomato seed germination and also generated high levels of malondialdehyde (MDA), O 2 - and H2O2. However, bioremediated ADOR did not negatively affect germination and reduced MDA, O 2 - and H2O2 content in different ways depending on the fungus used. In addition, the induced defense response was studied by analyzing the activity of both antioxidant enzymes (superoxide dismutase (SOD), catalase, ascorbate peroxidasa, glutathione reductase (GR), peroxidases and coniferil alcohol peroxidasa) and detoxification enzymes (glutathione-S-transferase (GST)). Our findings suggest that, because ADOR is capable of inducing oxidative stress, tomato seedlings trigger a defense response through SOD, GR, and GST activity and through antioxidant and lignification processes. On the other hand, the bioremediation of ADOR plays an important role in counteracting the oxidative stress induced by the untreated residue. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect