Decreasing dietary linoleic acid promotes long chain omega-3 fatty acid incorporation into rat retina and modifies gene expression

Autor: Stéphane Grégoire, Lionel Bretillon, Catherine Creuzot-Garcher, Niyazi Acar, Bertrand Bardet, Emilie Simon, Alain M. Bron
Přispěvatelé: Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Service d'Ophtalmologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), INRA (Human Nutrition Department, France), Laboratoires Horus Pharma (Saint Laurent du Var, France) 29000305, Regional Council of Burgundy (France) 29000362
Rok vydání: 2011
Předmět:
CD36 Antigens
Male
MESH : RNA
Messenger
MESH: 5-Lipoxygenase-Activating Proteins
MESH : Receptors
LDL
MESH: Electroretinography
0302 clinical medicine
MESH: Fatty Acids
Omega-3
MESH: Animals
MESH : Retinal Ganglion Cells
chemistry.chemical_classification
0303 health sciences
MESH : Gene Expression Regulation
MESH : Electroretinography
MESH: Retina
MESH: Chromatography
Gas
MESH: Dietary Fats
Unsaturated
docosahexaenoic acid
polyunsaturated fatty acid
Sensory Systems
3. Good health
nutrition
MESH: Photic Stimulation
Adipose Tissue
MESH: Adipose Tissue
medicine.medical_specialty
Chromatography
Gas
macular degeneration
Linoleic acid
MESH : Arachidonate 12-Lipoxygenase
Arachidonate 12-Lipoxygenase
MESH : Adipose Tissue
MESH: Arachidonate 12-Lipoxygenase
pufa
03 medical and health sciences
MESH : Dietary Fats
Unsaturated
lipid
Electroretinography
Rats
Long-Evans
RNA
Messenger
MESH: Linoleic Acid
MESH: Antigens
CD36
MESH : Retina
Fatty acid
MESH: Retinal Ganglion Cells
eye diseases
Ophthalmology
Endocrinology
chemistry
MESH: Receptors
LDL
030221 ophthalmology & optometry
ATP-Binding Cassette Transporters
n 3
MESH: Female
Photic Stimulation
MESH: Liver
Retinal Ganglion Cells
retina
MESH : 5-Lipoxygenase-Activating Proteins
genetic structures
[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition
retinal pigment epithelium
electroretinogram
MESH : Photic Stimulation
Adipose tissue
angiogenesis
chemistry.chemical_compound
MESH : Female
MESH : Rats
Long-Evans
2. Zero hunger
medicine.diagnostic_test
MESH : Rats
MESH: Real-Time Polymerase Chain Reaction
MESH: Gene Expression Regulation
MESH : Antigens
CD36
medicine.anatomical_structure
Liver
ALOX12
Biochemistry
MESH: ATP-Binding Cassette Transporters
Female
ATP Binding Cassette Transporter 1
Polyunsaturated fatty acid
MESH : Fatty Acids
Omega-3
MESH: Rats
brain
MESH : Male
5-Lipoxygenase-Activating Proteins
MESH : Real-Time Polymerase Chain Reaction
rhesus monkey
Biology
Real-Time Polymerase Chain Reaction
MESH : Chromatography
Gas
Linoleic Acid
Cellular and Molecular Neuroscience
Dietary Fats
Unsaturated
MESH : Linoleic Acid
MESH: Rats
Long-Evans
Internal medicine
Fatty Acids
Omega-3
medicine
Animals
MESH : ATP-Binding Cassette Transporters
Omega 3 fatty acid
MESH: RNA
Messenger
030304 developmental biology
deficient diet
Retinal pigment epithelium
MESH : Liver
MESH: Male
Rats
Gene Expression Regulation
Receptors
LDL
gene expression
MESH : Animals
sense organs
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Zdroj: Experimental Eye Research
Experimental Eye Research, Elsevier, 2011, 93 (5), pp.628-35. ⟨10.1016/j.exer.2011.07.016⟩
Experimental Eye Research, Elsevier, 2011, 93 (5), pp.628-35. 〈10.1016/j.exer.2011.07.016〉
ISSN: 0014-4835
1096-0007
DOI: 10.1016/j.exer.2011.07.016
Popis: International audience; Age-related macular degeneration (AMD) may be partially prevented by dietary habits privileging the consumption of ω3 long chain polyunsaturated fatty acids (ω3s) while lowering linoleic acid (LA) intake. The present study aimed to document whether following these epidemiological guidelines would enrich the neurosensory retina and RPE with ω3s and modulate gene expression in the neurosensory retina. Rat progenitors and pups were fed with diets containing low or high LA, and low or high ω3s. After scotopic single flash and 8-Hz-Flicker electroretinography, rat pups were euthanized at adulthood. The fatty acid profile of the neurosensory retina, RPE, liver, adipose tissue and plasma was analyzed using gas chromatography. Gene expression was analyzed with real-time PCR in the neurosensory retina. Diets rich in ω3s efficiently improved the incorporation of ω3s into the organs and tissues. This raising effect was magnified by lowering LA intake. Compared to a diet with high LA and low ω3s, low LA diets significantly upregulated LDL-receptor gene expression. Similar but not significant upregulation of CD36, ABCA1, ALOX5 and ALOX12 gene expression was observed in rats fed with low LA. No effect was observed on retinal function. Increasing the intake in ω3s and lowering LA improved the enrichment with ω3s of the tissues, including the neurosensory retina and RPE, and upregulated genes involved in lipid trafficking in the neurosensory retina. Those results consistently reinforced the beneficial role of ω3s in the prevention of AMD, especially when the diet contained low levels of LA, as suggested from epidemiological data.
Databáze: OpenAIRE
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