Non-radioactive chemical sequencing of biotin labelled DNA
| Autor: | Peter Richterich |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Streptavidin
Electrophoresis Chemical Phenomena Propanols Oligonucleotide Primer Maxam–Gilbert sequencing Biotin Biology DNA sequencing chemistry.chemical_compound Bacterial Proteins Genetics Base Sequence Oligonucleotide Organothiophosphates DNA Molecular biology Sequencing by ligation Restriction enzyme Chemistry Biochemistry chemistry Epoxy Compounds Colorimetry Single molecule real time sequencing |
| Zdroj: | Nucleic acids research. 17(6) |
| ISSN: | 0305-1048 |
| Popis: | Methods for the nonradioactive chemical sequencing of DNA are described. A biotin marker molecule, attached chemically to an oligonucleotide primer or enzymatically in an endfilling reaction of restriction enzyme sites, is stable during the base-specific chemical modification and strand scission reactions. Following fragment separation by direct blotting electrophoresis, the membrane bound sequence pattern can be visualized by a streptavidin-bridged enzymatic color reaction. The biotin labeling is also applicable for DNA sequencing by random degradation of phosphothioates, thus showing to be a universal label for nonradioactive DNA sequencing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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