Optical detection of the magnetophoretic transport of superparamagnetic beads on a micromagnetic array
| Autor: | Stefano Rampini, Dhruv Gandhi, Charlotte Parent, Gil U. Lee, Peng Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Materials science lcsh:Medicine 02 engineering and technology Bead Article 03 medical and health sciences Magnetization Engineering Nanoscience and technology Sensitivity (control systems) lcsh:Science Micromagnetics Multidisciplinary Orientation (computer vision) Dynamic range business.industry lcsh:R 021001 nanoscience & nanotechnology Magnetic field 030104 developmental biology Optics and photonics visual_art visual_art.visual_art_medium Optoelectronics lcsh:Q 0210 nano-technology business Biosensor Biotechnology |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Micromagnetic arrays (MMAs) have proven to be powerful tools for controlling the transport and separation of bioanalytes, i.e., they allow bioanalyte-superparamagnetic (SPM) bead complexes of specific size and magnetization to be moved in a synchronized manner that is precisely controlled with the orientation of an external magnetic field. This article presents a laser-photodetector system for the simple detection of individual SPM beads moving on a specific region of an MMA. This system detects the SPM beads through the change in intensity of reflective light as they move from the highly reflective micromagnetics to the supporting substrate. We demonstrate that this opti-MMA system allowed the size, number, and magnetic and optical properties of the SPM beads to be rapidly determined for regions > 49 µm2 in size. The response of the opti-MMA system was characterized in several optical configurations to develop a theoretical description of its sensitivity and dynamic range. The speed, low-cost, and sensitivity of this system promises to allow MMAs to be readily applied in in vitro diagnostics and biosensing. |
| Databáze: | OpenAIRE |
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