Molecular prevalence of Cryptosporidium isolates among Egyptian children with cancer
| Autor: | Sara A. Abdel-Rahman, Samira Metwally Mohammad, Raghda Abdelrahman Moustafa, Marwa A. Salama, Magda Saad Ali |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Cryptosporidium infection biology business.industry 030231 tropical medicine Cancer Cryptosporidium 030108 mycology & parasitology Malignancy medicine.disease biology.organism_classification 03 medical and health sciences Diarrhea 0302 clinical medicine Polymorphism (computer science) Internal medicine parasitic diseases Genotype medicine Parasitology Original Article medicine.symptom business Nested polymerase chain reaction |
| Zdroj: | J Parasit Dis |
| Popis: | Immunocompromised individuals especially children with cancer are at risk for acquiring cryptosporidiosis, which can result in severe morbidity and mortality. This work was conducted to evaluate the prevalence of Cryptosporidium parasite and its genotypes in children with cancer. Stool specimens were collected from 145 children in the Oncology unit of Pediatric Department, Zagazig University Hospital, Sharqiyah province, Egypt. Cryptosporidium infection was evaluated using modified Ziehl-Neelsen (MZN) staining and nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The prevalence of Cryptosporidium infection in oncological children was 29.7% using microscopy and 25.5% using nested PCR. Genotypic characterization showed that 23 (62.2%) had C. hominis, 11 (29.7%) C. parvum, and 3 specimens (8.1%) were mixed infection of both genotypes. Cryptosporidiosis was significantly associated with diarrhea. However, no statistically significant difference was detected between the age, gender, residency, animal contact and malignancy type concerning to Cryptosporidium infection. This study concluded that Cryptosporidium is a prevailing opportunistic parasite among children with cancer. It should be considered in oncological patients especially those suffering from diarrhea which requires proper management to reduce its complications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|