GFAP isoforms control intermediate filament network dynamics, cell morphology, and focal adhesions
| Autor: | Liselot J. Kluivers, Miriam E. van Strien, Thomas Schmidt, Vincent W. N. van der Meer, Martina Moeton, Elly M. Hol, Hedde van Hoorn, Oscar M. J. A. Stassen, Jacqueline A. Sluijs, Eric Reits |
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| Přispěvatelé: | Cellular and Computational Neuroscience (SILS, FNWI), Soft Tissue Biomech. & Tissue Eng., Medical Biochemistry, ANS - Cellular & Molecular Mechanisms, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Cell Biology and Histology, Netherlands Institute for Neuroscience (NIN) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Adult
0301 basic medicine Cell Survival Green Fluorescent Proteins Astrocytoma Cell morphology Microtubules Nestin Focal adhesion 03 medical and health sciences Cellular and Molecular Neuroscience Imaging Three-Dimensional Cell Movement Cell Line Tumor Glial Fibrillary Acidic Protein Journal Article medicine Humans Protein Isoforms Vimentin Intermediate filaments Intermediate filament Cell Shape Molecular Biology Actin Aged Cell Proliferation Pharmacology Focal Adhesions Glial fibrillary acidic protein biology GFAP Fluorescence recovery after photobleaching Cell migration Cell Biology Actins Cell biology 030104 developmental biology medicine.anatomical_structure Astrocytes FRAP biology.protein Molecular Medicine Original Article Female Astrocyte |
| Zdroj: | CELLULAR AND MOLECULAR LIFE SCIENCES CELLULAR AND MOLECULAR LIFE SCIENCES, 73(21), 4101-4120 Cellular and Molecular Life Sciences, 73(21), 4101-4120. Birkhauser Verlag Basel Cellular and Molecular Life Sciences Cellular and Molecular Life Sciences, 73(21). Birkhäuser Verlag Cellular and molecular life sciences, 73(21), 4101-4120. Birkhauser Verlag Basel Cellular and Molecular Life Sciences, 73(21), 4101–4120. Birkhauser Verlag Basel Cellular and Molecular Life Sciences, 73, 4101-4120. Birkhäuser Verlag |
| ISSN: | 1420-682X |
| Popis: | Glial fibrillary acidic protein (GFAP) is the characteristic intermediate filament (IF) protein in astrocytes. Expression of its main isoforms, GFAPα and GFAPδ, varies in astrocytes and astrocytoma implying a potential regulatory role in astrocyte physiology and pathology. An IF-network is a dynamic structure and has been functionally linked to cell motility, proliferation, and morphology. There is a constant exchange of IF-proteins with the network. To study differences in the dynamic properties of GFAPα and GFAPδ, we performed fluorescence recovery after photobleaching experiments on astrocytoma cells with fluorescently tagged GFAPs. Here, we show for the first time that the exchange of GFP–GFAPδ was significantly slower than the exchange of GFP–GFAPα with the IF-network. Furthermore, a collapsed IF-network, induced by GFAPδ expression, led to a further decrease in fluorescence recovery of both GFP–GFAPα and GFP–GFAPδ. This altered IF-network also changed cell morphology and the focal adhesion size, but did not alter cell migration or proliferation. Our study provides further insight into the modulation of the dynamic properties and functional consequences of the IF-network composition. Electronic supplementary material The online version of this article (doi:10.1007/s00018-016-2239-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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