The response of Lactococcus lactis to membrane protein production
| Autor: | Joao Coelho Pinto, Hjalmar P. Permentier, Eric R. Geertsma, Ravi K. R. Marreddy, Bert Poolman, Oscar P. Kuipers, Fabrizia Fusetti, Jan Kok, Justina C. Wolters |
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| Přispěvatelé: | Groningen Biomolecular Sciences and Biotechnology, Zernike Institute for Advanced Materials, Molecular Genetics, Analytical Biochemistry, Medicinal Chemistry and Bioanalysis (MCB) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Proteomics
STRESS Gene Expression lcsh:Medicine Biology Biochemistry Microbiology ACID BACTERIA 03 medical and health sciences Bacterial Proteins DNA-MICROARRAY DATA QUALITY-CONTROL Microbial Physiology Molecular Cell Biology Protein biosynthesis Genetics Overproduction lcsh:Science STRINGENT RESPONSE Integral membrane protein 030304 developmental biology Cellular Stress Responses 0303 health sciences Multidisciplinary 030306 microbiology Lactococcus lactis lcsh:R Membrane Proteins Bacteriology ABC TRANSPORTER OPUA Genomics Gene Expression Regulation Bacterial biology.organism_classification CONTROLLED GENE-EXPRESSION TRANSCRIPTOME ANALYSIS Membrane protein ESCHERICHIA-COLI Proteome Structural Genomics lcsh:Q Biogenesis SYSTEM Research Article |
| Zdroj: | PLoS ONE, 6(8):24060. PUBLIC LIBRARY SCIENCE PLoS ONE, Vol 6, Iss 8, p e24060 (2011) PLoS ONE PLoS ONE; Vol 6 |
| ISSN: | 1932-6203 |
| Popis: | Background: The biogenesis of membrane proteins is more complex than that of water-soluble proteins, and recombinant expression of membrane proteins in functional form and in amounts high enough for structural and functional studies is often problematic. To better engineer cells towards efficient protein production, we set out to understand and compare the cellular consequences of the overproduction of both classes of proteins in Lactococcus lactis, employing a combined proteomics and transcriptomics approach.Methodology and Findings: Highly overproduced and poorly expressed membrane proteins both resulted in severe growth defects, whereas amplified levels of a soluble substrate receptor had no effect. In addition, membrane protein overproduction evoked a general stress response (upregulation of various chaperones and proteases), which is probably due to accumulation of misfolded protein. Notably, upon the expression of membrane proteins a cell envelope stress response, controlled by the two-component regulatory CesSR system, was observed.Conclusions: The physiological response of L. lactis to the overproduction of several membrane proteins was determined and compared to that of a soluble protein, thus offering better understanding of the bottlenecks related to membrane protein production and valuable knowledge for subsequent strain engineering. |
| Databáze: | OpenAIRE |
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