Arthritis-induced increase in serum levels of IGF-binding protein-3 in rats is secondary to the decrease in its proteolytic activity
| Autor: | M.A. Villanúa, J. M. P. Holly, Ana Isabel Martín, Inmaculada Ibáñez de Cáceres, Asunción López-Calderón, Teresa Priego |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Inflammatory arthritis Proteolysis Arthritis Insulin-like growth factor-binding protein law.invention Endocrinology law Internal medicine Gene expression medicine Animals Humans RNA Messenger Insulin-Like Growth Factor I Rats Wistar Saline biology medicine.diagnostic_test business.industry medicine.disease Blotting Northern Arthritis Experimental Rats Insulin-Like Growth Factor Binding Protein 3 Liver Growth Hormone biology.protein Recombinant DNA business Adjuvant hormones hormone substitutes and hormone antagonists |
| Zdroj: | The Journal of endocrinology. 173(2) |
| ISSN: | 0022-0795 |
| Popis: | Adjuvant-induced arthritis is a chronic inflammatory illness that induces a catabolic state, with a decrease in pituitary GH and hepatic IGF-I synthesis. We have previously observed an increase in serum IGF-binding protein-3 (IGFBP-3) in arthritic rats, and found that GH administration prevents the increase in circulating IGFBP-3 in arthritic rats. The aim of this work was therefore to study IGFBP-3 synthesis in the liver as well as its proteolysis in serum as the two possible causes of the increased circulating IGFBP-3 in arthritic rats. The effect of recombinant human GH (rhGH) administration was also analysed. Adult male Wistar rats were injected with complete Freund's adjuvant or vehicle, and 14 days later they were injected s.c. daily until day 22 after adjuvant injection with rhGH (3 IU/kg) or saline. Three hours after the last GH injection, all rats were killed by decapitation. Arthritis increased serum IGFBP-3 levels (P |
| Databáze: | OpenAIRE |
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