Comparative effects of nacre water-soluble matrix and dexamethasone on the alkaline phosphatase activity of MRC-5 fibroblasts
| Autor: | Josiane Haigle, Evelyne Lopez, Maria Almeida, Lucilia Pereira, Mário A. Barbosa, Christian Milet |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Time Factors Stromal cell Biomedical Engineering Bone Morphogenetic Protein 2 Bone Marrow Cells Biology Bone morphogenetic protein 2 Dexamethasone Cell Line Biomaterials Transforming Growth Factor beta medicine Animals Humans Rats Wistar Fibroblast Glucocorticoids Dose-Response Relationship Drug MRC-5 Osteoblast Fibroblasts Alkaline Phosphatase Ostreidae Molecular biology Rats medicine.anatomical_structure Solubility Bone Morphogenetic Proteins Immunology Alkaline phosphatase Bone marrow Stem cell |
| Zdroj: | Journal of Biomedical Materials Research. 57:306-312 |
| ISSN: | 1097-4636 0021-9304 |
| DOI: | 10.1002/1097-4636(200111)57:2<306::aid-jbm1172>3.0.co;2-h |
| Popis: | In this study we demonstrate, for the first time, that dexamethasone and BMP-2 stimulated alkaline phosphatase (ALP) activity in MRC-5 fibroblasts, a cell line derived from human fetal lung. Previously we reported that the water-soluble matrix (WSM) of nacre obtained from the inner shell layer of the oyster Pinctada maxima, promoted an increase in ALP activity that was dose-dependent. In this work, we show that the effect of WSM is also time-dependent. As a comparison, the effect of WSM was also tested in bone marrow stromal cells because marrow and other bone surface-derived osteoblast stem cells have the inherent direct potential for osteogenesis. WSM promotes cell proliferation and ALP activity when tested with bone marrow cells in concentrations between 135 and 540 μg protein/mL. The effect of WSM on ALP activity of bone marrow stromal cells is similar to that obtained by dexamethasone. These results imply that MRC-5 fibroblasts respond to differentiating factors that promote osteoblastic phenotype in bone-derived cell cultures. © 2001 John Wiley & Sons, Inc. J Biomed Mater Res 57: 306–312, 2001 |
| Databáze: | OpenAIRE |
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