Process for an efficient lentiviral cell transduction

Autor:	Anna Chiara Pirona, Michael Boettcher, R. Oktriani, Jörg D. Hoheisel
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Methods Manuscript 0303 health sciences Cell type Computer science Cell Computational biology Genome General Biochemistry Genetics and Molecular Biology Small hairpin RNA 03 medical and health sciences Transduction (genetics) 0302 clinical medicine medicine.anatomical_structure Cell culture High complexity lentivirus shRNA 030220 oncology & carcinogenesis medicine CRISPR cell transduction CRISPR-Cas General Agricultural and Biological Sciences 030304 developmental biology
Zdroj:	Biology Methods & Protocols
ISSN:	2396-8923
Popis:	The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency of cell transduction. Low efficiencies with particular cell types and/or the high complexity of lentiviral libraries can cause insufficient representation. Here, we present a protocol that yielded substantial increases in transduction efficiency in various cell lines in comparison to several other procedures.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba89e8439a9e18a483b369880ca2a9e6 http://europepmc.org/articles/PMC7200879 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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