Lysophosphatidic acid accelerates development of porcine embryos by activating formation of the blastocoel
| Autor: | Yeo-Jin Son, Min-Young Shin, Eun-Young Kim, Se-Pill Park, Sang-Gi Jeong, Seung-Eun Lee, Yun-Gwi Park |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Swine Parthenogenesis Cell bcl-X Protein Embryonic Development Connexin Biology Cdh1 Proteins Embryo Culture Techniques Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lysophosphatidic acid Genetics medicine Animals Blastocyst 030219 obstetrics & reproductive medicine Blastocoel Embryogenesis Embryo Cell Biology Embryo Mammalian bcl-2 Homologous Antagonist-Killer Protein 030104 developmental biology medicine.anatomical_structure chemistry Apoptosis Connexin 43 embryonic structures lipids (amino acids peptides and proteins) Lysophospholipids biological phenomena cell phenomena and immunity Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 85:62-71 |
| ISSN: | 1040-452X |
| DOI: | 10.1002/mrd.22938 |
| Popis: | Culture media modifications, including the addition of various factors, are important for the in vitro production of oocytes and embryos. In this study, we investigated the effects of lysophosphatidic acid (LPA) on porcine embryo development. Porcine parthenogenetic embryos were cultured with 0, 0.1, 1, and 10 μM LPA for 7 days, or cultured in basic medium until Day 4 and then treated with LPA from Days 4 to 7. No difference in the in vitro development of embryos cultured with LPA for 7 days was observed. Conversely, rates of blastocyst and over-expanded blastocyst formation were higher in the 0.1 and 1 µM LPA-treated versus the other groups of embryos treated from Days 4 to 7. Moreover, formation of early blastocysts occurred earlier and embryo size was larger in LPA-treated compared to control embryos. Expression of Connexin 43 and gap junction and cell adhesion-related genes (GJC1 and CDH1, respectively) was also higher in LPA-treated compared to control embryos. Despite no difference in the blastocyst total cell number between groups, the apoptotic index was lower in the LPA-treated group than in the control group; indeed, BCL2L1 (B-cell lymphoma 2-like protein 1) expression increased while BAK (Bcl-2 homologous antagonist killer) decreased in the LPA-treated group. Thus, addition of LPA to the medium from Days 4 to 7 of culture improves blastocyst formation and aids the development of preimplantation embryos. |
| Databáze: | OpenAIRE |
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