Comparative Phenotypic and Genotypic Characterization of Temporally Related NontyphoidalSalmonellaIsolated from Human Clinical Cases, Pigs, and the Environment in North Carolina
| Autor: | Cami S Hartley, Wondwossen A. Gebreyes, Harvey Morgan Scott, Siddhartha Thakur, W. E. M. Morrow, Shivaramu Keelara, Denise Griffin |
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| Rok vydání: | 2014 |
| Předmět: |
Serotype
Salmonella Veterinary medicine Genotype Swine Tetracycline Microbial Sensitivity Tests Drug resistance Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology beta-Lactamases Integrons Bacterial Proteins Drug Resistance Multiple Bacterial North Carolina medicine Animals Humans Broth microdilution biochemical phenomena metabolism and nutrition Phenotype Anti-Bacterial Agents Cephalosporins Electrophoresis Gel Pulsed-Field Streptomycin bacteria Animal Science and Zoology Food Science medicine.drug |
| Zdroj: | Foodborne Pathogens and Disease. 11:156-164 |
| ISSN: | 1556-7125 1535-3141 |
| Popis: | Nontyphoidal Salmonella infections caused by antimicrobial-resistant (AMR) strains are of great public health concern. We compared the phenotypic and genotypic relationships among temporally and spatially related AMR Salmonella isolates (n=1058) representing several predominant serovars, including Salmonella Typhimurium, Salmonella Typhimurium var. 5-, Salmonella Derby, Salmonella Heidelberg, Salmonella Muenchen, Salmonella Schwarzengrund, and Salmonella Rissen of human clinical cases (n=572), pig (n=212), and farm environment (n=274) origin in North Carolina. Antimicrobial susceptibility testing was performed using the broth microdilution method, and genotypic resistance determinants, including class I and II integrons, were identified. Overall, Salmonella isolates exhibited the highest frequency of resistance to tetracycline (50%), followed by sulfisoxazole (36%) and streptomycin (27%). We identified 16 different antimicrobial resistance genes, including extended spectrum and AmpC β-lactamases-producing genes (bla(TEM), bla(PSE), and bla(CMY-2)), in all the β-lactam- and cephalosporin-resistant Salmonella isolates from humans, pigs, and the environment. Class I integrons of 1-kb and 1.2-kb size were identified from all the three sources (humans, 66%; pigs, 85%; environment, 58%), while Class II integrons of 2-kb size were identified only in pig (10%) and environmental (19%) isolates. We detected genotypic similarity between Salmonella Typhimurium isolated from humans, pigs, and the environment while serovars Derby, Heidelberg, and Muenchen exhibited genotypic diversity. Detection of AMR Salmonella isolates from humans, pigs, and the environment is a concern for clinicians and veterinarians to mitigate the dissemination of AMR Salmonella strains. |
| Databáze: | OpenAIRE |
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