Fasciola gigantica: Comparison of the tegumental ultrastructure in newly excysted metacercariae and in vitro penetrated juvenile flukes indicates intracellular sources of molecules with vaccinal and immunomodulatory potential
| Autor: | R.E.B. Hanna, Mark W. Robinson, W. G. Z. O. Jura, D. Moffett, Ian Fairweather, G.P. Brennan |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Fascioliasis Proteases Fasciola gigantica 030231 tropical medicine Exocytosis Glycocalyx 03 medical and health sciences 0302 clinical medicine Animals Immunologic Factors Metacercariae Vaccines Syncytium General Veterinary biology General Medicine 030108 mycology & parasitology Apical membrane biochemical phenomena metabolism and nutrition biology.organism_classification Fasciola Cell biology Cytoplasm Ultrastructure Parasitology Integumentary System |
| Zdroj: | Hanna, R E B, Moffett, D, Robinson, M W, Jura, W G Z O, Brennan, G P & Fairweather, I 2019, ' Fasciola gigantica: Comparison of the tegumental ultrastructure in newly excysted metacercariae and in vitro penetrated juvenile flukes indicates intracellular sources of molecules with vaccinal and immunomodulatory potential ', Veterinary Parasitology, vol. 265, pp. 38-47 . https://doi.org/10.1016/j.vetpar.2018.11.019 |
| Popis: | Using in vitro procedures to prepare newly excysted metacercariae and gut-penetrated juvenile Fasciola gigantica, the ultrastructural features of the tegumental syncytium and perikarya of these ephemeral stages in the host-invasion process were compared. The T0-type tegumental cells in newly excysted metacercariae are packed with stored T0 granules which, following transport to the surface membrane of the syncytium, discharge by exocytosis to maintain the glycocalyx. The T0 cells become depleted of T0 granules during the penetration process, shrink in size, and initiate autophagy in the cytoplasm to facilitate metamorphosis from a storage function to active biosynthesis. The novel products appear to include lysosomes which contribute to the autophagosomes, and T1 granules, necessary for maintenance of the glycocalyx and immunoprotection, as the invasion process continues into the host liver. Residual bodies, the end-products of autophagy, are eliminated from the apical membrane of the tegumental syncytium into the host-parasite interface. There they may present a transient source of parasite-derived molecules, including lysosomal cathepsin-type proteases, with potential for interaction with the host’s immune system, and so might be exploited as targets for vaccinal and immunomodulatory studies. |
| Databáze: | OpenAIRE |
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