The mRNA for an ETR1 homologue in tomato is constitutively expressed in vegetative and reproductive tissues
| Autor: | Dingbo Zhou, Panagiotis Kalaitzis, Mark L. Tucker, Autar K. Mattoo |
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| Rok vydání: | 1996 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Receptors Cell Surface Plant Science Biology Open Reading Frames Solanum lycopersicum Gene Expression Regulation Plant Complementary DNA Gene expression Genetics Coding region Amino Acid Sequence RNA Messenger Cloning Molecular Gene Peptide sequence Southern blot Plant Proteins Sequence Homology Amino Acid Nucleic acid sequence General Medicine Molecular biology Open reading frame Blotting Southern Biochemistry Agronomy and Crop Science Abscisic Acid |
| Zdroj: | Plant molecular biology. 30(6) |
| ISSN: | 0167-4412 |
| Popis: | Dominant mutations in the Arabidopsis ETR1 gene block the ethylene signal transduction pathway. The ETR1 gene has been cloned and sequenced. Using the ETR1 cDNA as a probe, we identified a cDNA homologue (eTAE1) from tomato. eTAE1 contains an open reading frame encoding a polypeptide of 754 amino acid residues. The nucleic acid sequence for the coding sequence in eTAE1 is 74% identical to that for ETR1, and the deduced amino acid sequence is 81% identical and 90% similar. Genomic Southern blot analysis indicates that three or more ETR1 homologues exist in tomato. RNA blots show that eTAE1 mRNA is constitutively expressed in all the tissues examined, and its accumulation in leaf abscission zones was unaffected by ethylene, silver ions (an inhibitor of ethylene action) or auxin. |
| Databáze: | OpenAIRE |
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