Effects of Vitamin A Deficiency and Repletion on Rat Glucagon Secretion
| Autor: | Henry K. Driscoll, W S Blaner, Mary Beth Cordle, Kimberly A. Matthews, Bruce S. Chertow, Paolo Meda |
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| Rok vydání: | 1994 |
| Předmět: |
Vitamin
Retinyl Esters endocrine system medicine.medical_specialty Arginine Receptors Retinoic Acid Endocrinology Diabetes and Metabolism Radioimmunoassay Tretinoin Biology Glucagon Alpha cell Cell Line Rats Sprague-Dawley Islets of Langerhans Retinoids chemistry.chemical_compound Endocrinology Pregnancy Internal medicine Insulin Secretion Internal Medicine medicine Animals Insulin Secretion Vitamin A Hepatology Vitamin A Deficiency Glucagon secretion Retinol Retinol-Binding Proteins Cellular medicine.disease Rats Retinol-Binding Proteins Vitamin A deficiency Glucose chemistry Female Diterpenes |
| Zdroj: | Pancreas. 9:475-484 |
| ISSN: | 0885-3177 |
| Popis: | To determine whether vitamin A is involved in pancreatic alpha cell function, we tested for (a) effects of vitamin A deficiency on glucagon release from perifused islets and perfused pancreases, and (b) the presence of cytosolic retinol-binding proteins (CRBP) and retinoic acid-binding proteins (CRABP), in the glucagon-secreting alpha cell line, ln-R1-G9. Arginine 19 mM plus glucose 2.8 mM-stimulated glucagon secretion was markedly impaired in islets and pancreases of vitamin A-deficient rats or rats that had at some time been cycled through vitamin A deficiency (ever A-def) despite repletion with retinoids for 2-4 weeks. Insulin secretion was impaired likewise. Repletion starting early in the development of vitamin A deficiency and for a longer period of time (18 or 60 days) did not restore glucagon secretion, but did normalize insulin secretion. CRBP and CRABP were present in ln-R1-G9 cells. We conclude that (a) vitamin A deficiency is associated with a defect in glucagon secretion; (b) The defect in secretion occurs early in the course of vitamin A deficiency; (c) The defect persists despite repletion; and (d) The requirement of vitamin A for secretion and the presence of CRBP and CRABP in glucagon-secreting cells support a physiologic role for vitamin A at the alpha cell level. |
| Databáze: | OpenAIRE |
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