Assessing the biofuel production potential of Botryococcus braunii strains by sensitive rapid qualitative chemotyping using chemometrically-assisted gas chromatography–mass spectrometry
| Autor: | Delphine Kucma, Marion André, Dominique Grizeau, Benjamin Moutel, Jeremy Pruvost, Olivier Gonçalves, Jack Legrand |
|---|---|
| Přispěvatelé: | Bioprocédés Appliqués aux Microalgues (GEPEA-BAM), Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)-Institut Universitaire de Technologie - Nantes (IUT Nantes), Université de Nantes (UN)-Institut Universitaire de Technologie Saint-Nazaire (IUT Saint-Nazaire), Université de Nantes (UN)-Institut Universitaire de Technologie - La Roche-sur-Yon (IUT La Roche-sur-Yon), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences Chromatography biology 010405 organic chemistry Chemistry biology.organism_classification 7. Clean energy 01 natural sciences 0104 chemical sciences Chemometrics 03 medical and health sciences Hydrocarbon [CHIM.GENI]Chemical Sciences/Chemical engineering Chemotaxonomy Biofuel [CHIM.ANAL]Chemical Sciences/Analytical chemistry Botryococcus braunii Selected ion monitoring Gas chromatography–mass spectrometry Agronomy and Crop Science Long chain 030304 developmental biology |
| Zdroj: | Algal Research-Biomass, Biofuels and Bioproducts Algal Research-Biomass, Biofuels and Bioproducts, Elsevier, 2015, 11, pp.33-42. ⟨10.1016/j.algal.2015.05.001⟩ |
| ISSN: | 2211-9264 |
| DOI: | 10.1016/j.algal.2015.05.001⟩ |
| Popis: | International audience; The microalga Botryococcus braunii has been studied for its hydrocarbon biofuel production potential. The range of the lipids it synthesizes cannot be readily profiled, because their main chemical class standards (long chain al-kenes and botryococcenes) are at present unavailable. The aim of this study was to develop a direct, specific GCMS analysis method for the rapid chemotyping of these lipids. The SIM (Selected Ion Monitoring)-GCMS program we developed discriminates classes and subclasses of the targeted molecules through their main chemical motifs identified with fragment ions of specific m/z selected by unsupervised chemometrics. Our validation results indicate that the SIM mode enhanced their detection by significantly increasing the signal-to-noise ratio ca. 20-fold. The profiling results from TL extracts of diverse B. braunii strains show that under the culture conditions tested, these strains could be distinguished by their chemical signatures, which also signaled different culture conditions involving various physiological states. We were able to make a rapid estimate of the biofuel potential of these strains, focusing on alkenes and botryococcenes. The direct injection SIM-GCMS method was successfully applied here for rapid chemotaxonomy, displaying good sensitivity and specificity for new high molecular weight hydrocarbons from B. braunii. |
| Databáze: | OpenAIRE |
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