Sultr4;1 mutant seeds of Arabidopsis have an enhanced sulphate content and modified proteome suggesting metabolic adaptations to altered sulphate compartmentalization
| Autor: | Maya Belghazi, Markus Wirtz, Richard D. Thompson, Jean-Claude Davidian, Rüdiger Hell, Hélène Zuber, Karine Gallardo |
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| Přispěvatelé: | Génétique et Ecophysiologie des Légumineuses à Graines (UMRLEG) (UMR 102), Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Heidelberg Institute of Plant Sciences, Universität Heidelberg [Heidelberg], Department of Plant Molecular Biology, Université de Lausanne (UNIL), Centre de recherche en neurobiologie - neurophysiologie de Marseille (CRN2M), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université de Lausanne = University of Lausanne (UNIL), Baracco, Chantal, Universität Heidelberg [Heidelberg] = Heidelberg University |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
0106 biological sciences
Proteome Mutant Sultr4 Plant Science Vacuole sulfate Proteomics vacuolar membrane 01 natural sciences MESH: Cell Compartmentation Gene Expression Regulation Plant transcript levels Arabidopsis MESH: Reverse Transcriptase Polymerase Chain Reaction lcsh:Botany MESH: Gene Expression Regulation Developmental [SDV.BV] Life Sciences [q-bio]/Vegetal Biology Electrophoresis Gel Two-Dimensional MESH: Arabidopsis MESH: Nitrogen protéome 2. Zero hunger chemistry.chemical_classification MESH: Glutathione 0303 health sciences Vegetal Biology biology Reverse Transcriptase Polymerase Chain Reaction Sulfates SULFUR SULFATE VACUOLAR MEMBRANE SULTR4 TRANSCRIPT LEVELS BIOLOGIE MOLECULAIRE BIOLOGIE DU DEVELOPPEMENT Gene Expression Regulation Developmental MESH: Anion Transport Proteins Compartmentalization (psychology) Adaptation Physiological Glutathione Amino acid lcsh:QK1-989 MESH: Plant Leaves MESH: Proteome Biochemistry Seeds Efflux Research Article DNA Bacterial MESH: Mutation expression génique Nitrogen Anion Transport Proteins MESH: Carbon Flowers MESH: Arabidopsis Proteins 03 medical and health sciences MESH: Gene Expression Profiling physiologie végétale [SDV.BV]Life Sciences [q-bio]/Vegetal Biology RNA Messenger MESH: Gene Expression Regulation Plant 030304 developmental biology MESH: RNA Messenger Arabidopsis Proteins Gene Expression Profiling biology.organism_classification MESH: Electrophoresis Gel Two-Dimensional MESH: Flowers MESH: Adaptation Physiological MESH: DNA Bacterial Carbon Cell Compartmentation Plant Leaves Mutagenesis Insertional arabidopsis chemistry MESH: Mutagenesis Insertional MESH: Seeds Mutation Biologie végétale 010606 plant biology & botany MESH: Sulfates |
| Zdroj: | BMC Plant Biology, Vol 10, Iss 1, p 78 (2010) BMC Plant Biology (10), 78. (2010) BMC Plant Biology BMC Plant Biology, BioMed Central, 2010, 10, pp.78. ⟨10.1186/1471-2229-10-78⟩ BMC Plant Biology, 2010, 10, pp.78. ⟨10.1186/1471-2229-10-78⟩ |
| ISSN: | 1471-2229 |
| DOI: | 10.1186/1471-2229-10-78⟩ |
| Popis: | Background Sulphur is an essential macronutrient needed for the synthesis of many cellular components. Sulphur containing amino acids and stress response-related compounds, such as glutathione, are derived from reduction of root-absorbed sulphate. Sulphate distribution in cell compartments necessitates specific transport systems. The low-affinity sulphate transporters SULTR4;1 and SULTR4;2 have been localized to the vacuolar membrane, where they may facilitate sulphate efflux from the vacuole. Results In the present study, we demonstrated that the Sultr4;1 gene is expressed in developing Arabidopsis seeds to a level over 10-fold higher than the Sultr4;2 gene. A characterization of dry mature seeds from a Sultr4;1 T-DNA mutant revealed a higher sulphate content, implying a function for this transporter in developing seeds. A fine dissection of the Sultr4;1 seed proteome identified 29 spots whose abundance varied compared to wild-type. Specific metabolic features characteristic of an adaptive response were revealed, such as an up-accumulation of various proteins involved in sugar metabolism and in detoxification processes. Conclusions This study revealed a role for SULTR4;1 in determining sulphate content of mature Arabidopsis seeds. Moreover, the adaptive response of sultr4;1 mutant seeds as revealed by proteomics suggests a function of SULTR4;1 in redox homeostasis, a mechanism that has to be tightly controlled during development of orthodox seeds. |
| Databáze: | OpenAIRE |
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