The presence of high-risk HPV combined with specific p53 and p16INK4a expression patterns points to high-risk HPV as the main causative agent for adenocarcinoma in situ and adenocarcinoma of the cervix
| Autor: | Feja J. Voorhorst, G. Denise Zielinski, Elle K.J. Risse, Peter J.F. Snijders, Nathalie Fransen Daalmeijer, Frits A. de Schipper, Lawrence Rozendaal, Hans van der Linden, Chris J.L.M. Meijer, Arnold P Runsink, N Medi Jiwa |
|---|---|
| Přispěvatelé: | VU University medical center |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Adult
Oncology medicine.medical_specialty Pathology Population Uterine Cervical Neoplasms Adenocarcinoma Biology Polymerase Chain Reaction Pathology and Forensic Medicine Risk Factors Internal medicine medicine Humans education Papillomaviridae Cervix education.field_of_study Genes p16 Carcinoma in situ Incidence (epidemiology) Papillomavirus Infections Anatomical pathology DNA Neoplasm Middle Aged Genes p53 medicine.disease Immunohistochemistry Endometrial Neoplasms Gene Expression Regulation Neoplastic medicine.anatomical_structure DNA Viral Female Carcinoma in Situ Immunostaining |
| Zdroj: | Zielinski, G D, Snijders, P J F, Rozendaal, L, Daalmeijer, NF, Risse, E K J, Voorhorst, F J, Jiwa, N M, van der Linden, H C, de Schipper, FA, Runsink, AP & Meijer, C J L M 2003, ' The presence of high-risk HPV combined with specific p53 and p16INK4a expression patterns points to high-risk HPV as the main causative agent for adenocarcinoma in situ and adenocarcinoma of the cervix. ', Journal of Pathology, vol. 201, no. 4, pp. 535-43 . https://doi.org/10.1002/path.1480 Journal of Pathology, 201(4), 535-43. John Wiley and Sons Ltd |
| ISSN: | 0022-3417 |
| DOI: | 10.1002/path.1480 |
| Popis: | Adenocarcinoma in situ (ACIS) and adenocarcinoma (AdCA) of the cervix are frequently missed in population-based screening programmes. Adding high-risk HPV (hrHPV) testing to cervical cancer screening might improve the detection rate of ACIS and AdCA. Since the exact proportion of AdCAs of the cervix that can be attributed to hrHPV infection is still a matter of debate, a comprehensive study was performed of hrHPV presence in ACIS and AdCA of the cervix. Archival formalin-fixed specimens of indisputable ACIS (n=65) and AdCA (n=77) of the cervix were tested for hrHPV DNA by GP5+/6+ PCR-enzyme immunoassay (EIA) and type-specific E7 PCR for 14 hrHPV types. Further immunostaining for p16INK4A and p53 was performed to assess alternative pathways of carcinogenesis potentially unrelated to HPV. hrHPV DNA was found in all (100%) ACISs and 72 (94%) cervical AdCAs, whereas none of 20 endometrial AdCAs scored hrHPV-positive. HPV 18 was most prevalent and found as single or multiple infection in 68% of ACISs and 55% of cervical AdCAs. Diffuse immunostaining for p16INK4a, a potential marker of hrHPV E7 function, was significantly more frequent in hrHPV-positive cervical AdCAs (19/20; 95%) than in those without hrHPV (1/5; 20%; p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text